Mito-TEMPO Demonstrates Protective Effect Against Ultraviolet Radiation-Induced Skin Damage in Wistar Rats.

IF 2.5 4区医学 Q2 DERMATOLOGY

Photodermatology, photoimmunology & photomedicine Pub Date : 2024-11-01 DOI:10.1111/phpp.13010

Sachin Shetty, Kingsly Deepak, Prasad Kisan Tambe, Anushree Udupi, Sanjay Bharati

{"title":"Mito-TEMPO Demonstrates Protective Effect Against Ultraviolet Radiation-Induced Skin Damage in Wistar Rats.","authors":"Sachin Shetty, Kingsly Deepak, Prasad Kisan Tambe, Anushree Udupi, Sanjay Bharati","doi":"10.1111/phpp.13010","DOIUrl":null,"url":null,"abstract":"Background: Mitochondria could be an important target for ultraviolet radiation (UVR)-induced skin damage. Therefore, protecting mitochondria using mitochondria-targeted antioxidants may protect skin from UV-induced photodamage.Methods: In the present study, UVR-induced skin damage model was developed by irradiating male Wistar rats with UVB at a dose of 120 mJ/cm2, twice a week for a period of 5 weeks. Mito-TEMPO was administered intraperitoneally (0.1 mg/kg b.w.) twice a week for 5 weeks. After 5 weeks of treatment period, animals were sacrificed and the dorsal skin tissues were collected. Physical examinations of the skin for analyzing wrinkle formation and epidermal thickening were carried out. Skin tissues were used for the evaluation of histopathological changes, mitochondrial dysfunction analysis, and mitochondrial membrane potential.Results: Physical and histological examination showed that mito-TEMPO protected from the damaging effect of UVB radiation. A significant increase in mitochondrial reactive oxygen species (mtROS) generation with a concomitant increase in mitochondrial lipid peroxidation (mtLPO) was observed in UV-irradiated groups. UV-induced generation of mtROS and mtLPO formation was effectively reduced by mito-TEMPO. Mito-TEMPO pre-treatment improved mitochondrial complex II activity, which was significantly reduced in the UV-irradiated group.Conclusion: The results suggested that mito-TEMPO effectively protected skin tissue against UV-induced oxidative stress and damage.","PeriodicalId":20123,"journal":{"name":"Photodermatology, photoimmunology & photomedicine","volume":"40 6","pages":"e13010"},"PeriodicalIF":2.5000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Photodermatology, photoimmunology & photomedicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/phpp.13010","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DERMATOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Mitochondria could be an important target for ultraviolet radiation (UVR)-induced skin damage. Therefore, protecting mitochondria using mitochondria-targeted antioxidants may protect skin from UV-induced photodamage.

Methods: In the present study, UVR-induced skin damage model was developed by irradiating male Wistar rats with UVB at a dose of 120 mJ/cm², twice a week for a period of 5 weeks. Mito-TEMPO was administered intraperitoneally (0.1 mg/kg b.w.) twice a week for 5 weeks. After 5 weeks of treatment period, animals were sacrificed and the dorsal skin tissues were collected. Physical examinations of the skin for analyzing wrinkle formation and epidermal thickening were carried out. Skin tissues were used for the evaluation of histopathological changes, mitochondrial dysfunction analysis, and mitochondrial membrane potential.

Results: Physical and histological examination showed that mito-TEMPO protected from the damaging effect of UVB radiation. A significant increase in mitochondrial reactive oxygen species (mtROS) generation with a concomitant increase in mitochondrial lipid peroxidation (mtLPO) was observed in UV-irradiated groups. UV-induced generation of mtROS and mtLPO formation was effectively reduced by mito-TEMPO. Mito-TEMPO pre-treatment improved mitochondrial complex II activity, which was significantly reduced in the UV-irradiated group.

Conclusion: The results suggested that mito-TEMPO effectively protected skin tissue against UV-induced oxidative stress and damage.

查看原文本刊更多论文

Mito-TEMPO 对紫外线辐射诱发的 Wistar 大鼠皮肤损伤具有保护作用。

背景：线粒体可能是紫外线辐射（UVR）诱导的皮肤损伤的一个重要靶点。因此，使用线粒体靶向抗氧化剂保护线粒体可保护皮肤免受紫外线诱导的光损伤：本研究建立了紫外线诱导的皮肤损伤模型，用 120 mJ/cm2 剂量的 UVB 照射雄性 Wistar 大鼠，每周两次，为期 5 周。腹腔注射米托-TEMPO（0.1 毫克/千克体重），每周两次，连续 5 周。治疗 5 周后，动物被处死并收集背侧皮肤组织。对皮肤进行物理检查，分析皱纹形成和表皮增厚情况。皮肤组织用于评估组织病理学变化、线粒体功能障碍分析和线粒体膜电位：物理和组织学检查表明，丝裂霉素能保护皮肤免受紫外线的伤害。紫外线照射组的线粒体活性氧（mtROS）生成量明显增加，线粒体脂质过氧化物（mtLPO）也随之增加。紫外线诱导的 mtROS 生成和 mtLPO 形成可通过丝裂霉素-TEMPO 有效减少。在紫外线照射组中，线粒体复合体II的活性显著降低，而在紫外线照射组中，线粒体复合体II的活性显著提高：结果表明，mito-TEMPO 能有效保护皮肤组织免受紫外线引起的氧化应激和损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Photodermatology, photoimmunology & photomedicine 医学-皮肤病学

CiteScore

4.40

自引率

7.70%

发文量

审稿时长

6-12 weeks

期刊介绍： The journal is a forum for new information about the direct and distant effects of electromagnetic radiation (ultraviolet, visible and infrared) mediated through skin. The divisions of the editorial board reflect areas of specific interest: aging, carcinogenesis, immunology, instrumentation and optics, lasers, photodynamic therapy, photosensitivity, pigmentation and therapy. Photodermatology, Photoimmunology & Photomedicine includes original articles, reviews, communications and editorials. Original articles may include the investigation of experimental or pathological processes in humans or animals in vivo or the investigation of radiation effects in cells or tissues in vitro. Methodology need have no limitation; rather, it should be appropriate to the question addressed.