Biochanin A Attenuates Psoriasiform Inflammation by Regulating Nrf2/HO-1 Pathway Activation and Attenuating Inflammatory Signalling.

IF 1.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yaping Lv, Yingsheng Xu, Songchun Liu, Xianjing Zeng, Bin Yang
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引用次数: 0

Abstract

Psoriasis is a long-term inflammatory skin condition marked by an overabundance of keratinocytes and the release of pro-inflammatory cytokines in the outer layer of skin. For the comprehensive management of intermediate to advanced psoriasis, innovative biological treatments have been developed. Products for the superficial therapy of mild to moderate psoriasis are still necessary, though. Trifolium pratense contains the isoflavone biochanin A (BCA), which exhibits antiviral, antioxidant, anti-carcinogenic, and anti-inflammatory properties, and helps protect the integrity and function of the endothelium. Although investigations have not shown that BCA is effective in treating psoriasis, it has been shown to slow down the breakdown of the skin barrier by regulating keratinocyte growth. We sought to clarify the basic mechanisms behind BCA's impact on psoriasis in vitro and in vivo using experimental research via regulating Nrf2/HO-1 signaling pathway. By subjecting human primary keratinocytes to psoriasis-related cytokines, psoriasis-like keratinocytes were produced. The CCK8 test was used in this investigation to assess cell viability. BCA reduced keratinocyte growth and inflammatory cascade stimulation produced by TNF-α and IL-6, according to in vitro investigations conducted on HaCaT cells. The in vivo findings showed that six days of BCA therapy significantly decreased the skin, hematological indicators, levels of NO, TBARS, histopathological, and pro-inflammatory factors of COX-2, iNOS, NF-κB pathway. It additionally influenced the protein content of pro-inflammatory cytokines such as IL-17, IL-23, IL-1β in the epidermis along with IL-6, TNF-α among the epidermis and serum. In addition, in contrast to the IMQ group, BCA improved the skin's level of Nrf2/HO-1 protein, anti-inflammatory cytokine IL-10, and antioxidant indicators like SOD, CAT, GST, GSH, GR, and Vit-C. Ultimately, our research shows that BCA was effective in treating psoriasis in pre-clinical animal models by activating the Nrf2/HO-1 pathway, leading to an increase in antioxidant and anti-inflammatory markers.

生物钱币素 A 通过调节 Nrf2/HO-1 通路活化和抑制炎症信号来减轻牛皮癣炎症反应
牛皮癣是一种长期炎症性皮肤病,其特征是皮肤外层角质细胞过多并释放促炎症细胞因子。为了全面治疗中晚期银屑病,我们开发了创新的生物疗法。不过,用于轻度至中度银屑病表层治疗的产品仍然是必要的。三叶草含有异黄酮生物碱 A(BCA),具有抗病毒、抗氧化、抗癌和抗炎特性,有助于保护血管内皮的完整性和功能。虽然研究并未显示 BCA 能有效治疗牛皮癣,但它已被证明能通过调节角质细胞的生长来减缓皮肤屏障的破坏。我们试图通过调节 Nrf2/HO-1 信号通路的实验研究,阐明 BCA 在体外和体内对银屑病产生影响的基本机制。将人类原代角朊细胞置于银屑病相关细胞因子的作用下,可产生类似银屑病的角朊细胞。这项研究使用 CCK8 试验来评估细胞活力。根据对 HaCaT 细胞进行的体外研究,BCA 可减少角质细胞的生长以及 TNF-α 和 IL-6 产生的炎症级联刺激。体内研究结果表明,六天的 BCA 治疗显著降低了皮肤、血液学指标、NO 水平、TBARS、组织病理学以及 COX-2、iNOS、NF-κB 通路的促炎因子。此外,它还影响了表皮中 IL-17、IL-23、IL-1β 等促炎细胞因子的蛋白质含量,以及表皮和血清中 IL-6、TNF-α 的含量。此外,与 IMQ 组相比,BCA 提高了皮肤中 Nrf2/HO-1 蛋白、抗炎细胞因子 IL-10 以及 SOD、CAT、GST、GSH、GR 和 Vit-C 等抗氧化指标的水平。最终,我们的研究表明,BCA 可通过激活 Nrf2/HO-1 通路,增加抗氧化和抗炎指标,从而有效治疗临床前动物模型中的银屑病。
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来源期刊
Cell Biochemistry and Biophysics
Cell Biochemistry and Biophysics 生物-生化与分子生物学
CiteScore
4.40
自引率
0.00%
发文量
72
审稿时长
7.5 months
期刊介绍: Cell Biochemistry and Biophysics (CBB) aims to publish papers on the nature of the biochemical and biophysical mechanisms underlying the structure, control and function of cellular systems The reports should be within the framework of modern biochemistry and chemistry, biophysics and cell physiology, physics and engineering, molecular and structural biology. The relationship between molecular structure and function under investigation is emphasized. Examples of subject areas that CBB publishes are: · biochemical and biophysical aspects of cell structure and function; · interactions of cells and their molecular/macromolecular constituents; · innovative developments in genetic and biomolecular engineering; · computer-based analysis of tissues, cells, cell networks, organelles, and molecular/macromolecular assemblies; · photometric, spectroscopic, microscopic, mechanical, and electrical methodologies/techniques in analytical cytology, cytometry and innovative instrument design For articles that focus on computational aspects, authors should be clear about which docking and molecular dynamics algorithms or software packages are being used as well as details on the system parameterization, simulations conditions etc. In addition, docking calculations (virtual screening, QSAR, etc.) should be validated either by experimental studies or one or more reliable theoretical cross-validation methods.
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