An Antibiofouling Electrochemical Sensor Consisting of Amphiphilic Copolymer on CNT-COOH Electrodes for Point-of-Care Monitoring of Propofol Blood Concentration in Surgical Patients

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2024-11-05 DOI:10.1016/j.snb.2024.136890

Shan Wang, Chunguang Kuai, Qianwen He, Feng He, Bingrui Xiong, Li Li, Xue Ke, Yuzheng Guo, Mian Peng

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Abstract

The monitoring of propofol (PPF) blood concentration is vital for the safety of intravenous anesthesia. Electrochemical sensors represent a powerful tool for the detection of PPF. Our previous research has demonstrated that CNT-COOH electrodes exhibit advantages in resisting electrochemical fouling from PPF oxidation products. Nevertheless, biofouling remains a critical challenge hindering the clinical transformation of PPF electrochemical sensors. To address this issue, we employed in-situ electrodeposition to deposit zwitterionic sulfobetaine methacrylate (SBMA) and overoxidation polydopamine (OPDA) onto CNT-COOH screen-printed electrodes (SPEs) and proposed an innovative CNT-COOH/OPDA-SBMA (CNT-COOH/OPS) amphiphilic antifouling biosensor. This amphiphilic electrode, featuring both hydrophilic and hydrophobic properties, demonstrated excellent antifouling performance through electrochemical characterization in ex-vivo blood assays, coupled with commendable sensitivity, stability, and selectivity. CNT-COOH/OPS SPEs were also applied in point-of-care monitoring of the PPF blood concentrations in animals and clinical surgical patients and exhibited good accuracy and linearity (Ipa=0.0084c+0.4458, R²=0.995) with a limit of detection of 2.948 μM. Further, the electrodes demonstrated a high recovery and the blood concentrations determined by electrochemical detection had robust negative correlation with the depth of anesthesia. Therefore, CNT-COOH/OPS SPEs hold promise as a significant tool in point-of-care monitoring of PPF, offering patients safer and more personalized medical care.

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一种由 CNT-COOH 电极上的两性共聚物组成的抗生物污染电化学传感器，用于对手术患者的异丙酚血药浓度进行床旁监测

监测异丙酚（PPF）的血液浓度对静脉麻醉的安全性至关重要。电化学传感器是检测丙泊酚的有力工具。我们之前的研究表明，CNT-COOH 电极在抵抗 PPF 氧化产物造成的电化学污垢方面具有优势。然而，生物污垢仍然是阻碍 PPF 电化学传感器临床转化的关键挑战。为了解决这个问题，我们采用原位电沉积的方法，在 CNT-COOH 丝网印刷电极（SPE）上沉积了齐聚物甲基丙烯酸磺基甜菜碱（SBMA）和过氧化多巴胺（OPDA），并提出了一种创新的 CNT-COOH/OPDA-SBMA （CNT-COOH/OPS）两亲性防污生物传感器。这种兼具亲水和疏水特性的两亲电极在体内外血液检测的电化学表征中表现出卓越的防污性能，同时还具有值得称道的灵敏度、稳定性和选择性。CNT-COOH/OPS SPEs 还被应用于动物和临床手术患者血液中 PPF 浓度的床旁监测，表现出良好的准确性和线性（Ipa=0.0084c+0.4458，R2=0.995），检测限为 2.948 μM。此外，电极的恢复能力很强，通过电化学检测确定的血液浓度与麻醉深度呈稳健的负相关。因此，CNT-COOH/OPS SPEs 有望成为床旁监测 PPF 的重要工具，为患者提供更安全、更个性化的医疗服务。

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.