Source attribution of Listeria monocytogenes in the Netherlands

IF 5 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY
Lapo Mughini-Gras , Julian A. Paganini , Ruoshui Guo , Claudia E. Coipan , Ingrid H.M. Friesema , Angela H.A.M. van Hoek , Maaike van den Beld , Sjoerd Kuiling , Indra Bergval , Bart Wullings , Menno van der Voort , Eelco Franz , Timothy J. Dallman
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Abstract

The aim of this study was to determine the relative contributions of various potential food sources of human listeriosis and to identify source-specific risk factors, at exposure level, for human Listeria monocytogenes (Lm) infection. To achieve this, available Lm isolates from human cases (n = 756) and food/animal sources (n = 950) from national surveillance systems in the Netherlands (2010−2020) were whole genome sequenced. Additionally, questionnaire-based exposure data for human cases was collected. Source attribution analysis was performed using a Random Forest model based on core-genome multilocus sequence typing (cgMLST). Risk factors for human Lm infection of cattle, chicken and seafood origin were determined using beta regression analysis on the cgMLST-based attribution estimates. Results indicated that the 756 human Lm isolates were mainly attributed to cattle (62.3 %), chicken (19.4 %), and seafood (16.9 %). Specifically, fresh meat (86.2 %), including fresh bovine meat (43.7 %) and fresh chicken meat (39.3 %), accounted for most cases. These attributions stemmed from Lm contamination of either the food products or their production environments. Consumption of steak tartare and smoked salmon was associated with an increased risk of human Lm infections attributed to cattle and seafood, respectively, while no specific risk factors for chicken-borne listeriosis were identified. This study indicated that Lm isolates of cattle origin, particularly those from fresh bovine meat and associated production environments, are estimated to be the primary cause of human listeriosis in the Netherlands. This aligns with several other European source attribution studies on Lm. Moreover, the identified risk factors for human Lm infection from cattle (i.e. steak tartare) and seafood (i.e. smoked salmon) clearly indicated their attributable sources. This joint analysis of core genome and epidemiological data provided novel insights into the origins and transmission pathways of human listeriosis.
荷兰李斯特菌的来源归属
本研究旨在确定各种潜在食物来源对人类李斯特菌病的相对贡献,并在暴露水平上确定人类李斯特菌(Lm)感染的特定来源风险因素。为此,对来自荷兰国家监测系统(2010-2020 年)的人类病例(n = 756)和食物/动物来源(n = 950)的 Lm 分离物进行了全基因组测序。此外,还收集了人类病例的问卷接触数据。使用基于核心基因组多焦点序列分型(cgMLST)的随机森林模型进行了来源归因分析。通过对基于 cgMLST 的归因估计值进行贝塔回归分析,确定了人类感染牛、鸡和海鲜来源 Lm 的风险因素。结果表明,756 个人类 Lm 分离物主要来自牛(62.3%)、鸡(19.4%)和海鲜(16.9%)。具体来说,新鲜肉类(86.2%),包括新鲜牛肉(43.7%)和新鲜鸡肉(39.3%)占大多数。这些病例都是由于食品或其生产环境受到 Lm 污染所致。食用鞑靼牛排和熏三文鱼分别与牛和海鲜导致人类感染李氏杆菌的风险增加有关,而鸡肉传播的李氏杆菌病没有发现特定的风险因素。这项研究表明,据估计,在荷兰,牛源性 Lm 分离物,尤其是来自新鲜牛肉和相关生产环境的分离物,是导致人类李斯特菌病的主要原因。这与欧洲其他几项关于李氏杆菌来源的研究结果一致。此外,从牛(即鞑靼牛排)和海鲜(即熏鲑鱼)中确定的人类李氏杆菌感染风险因素明确表明了其可归因来源。通过对核心基因组和流行病学数据的联合分析,我们对人类李斯特菌病的起源和传播途径有了新的认识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
International journal of food microbiology
International journal of food microbiology 工程技术-食品科技
CiteScore
10.40
自引率
5.60%
发文量
322
审稿时长
65 days
期刊介绍: The International Journal of Food Microbiology publishes papers dealing with all aspects of food microbiology. Articles must present information that is novel, has high impact and interest, and is of high scientific quality. They should provide scientific or technological advancement in the specific field of interest of the journal and enhance its strong international reputation. Preliminary or confirmatory results as well as contributions not strictly related to food microbiology will not be considered for publication.
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