Effect of green and UVA spectra, and pre-harvest treatments on biomass and metabolite yields of indoor cultivated stevia rebaudiana

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-11-02 DOI:10.1016/j.plaphy.2024.109252

Narendren Rengasamy , Rofina Yasmin Othman , Hang Seng Che , Jennifer Ann Harikrishna

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Abstract

Stevia rebaudiana is a high-value crop due to the strong commercial demand for its metabolites (steviol glycosides, SG) as an organic low-caloric sweetener with up to 300 times the sweetness of conventional sugar. Two experiments were conducted in this study. In the first experiment, treatments with varying green (GR1 & GR2), UVA (UV1 & UV2) and treatments that had both (UVGR1, UVGR2) were used. In the second experiment, separate set of plants were grown under base red-blue (RB) and natural sunlight before being transferred to GR2, UV2, UVGR2, and monochromatic light treatments of blue, green and UVA, for 3 and 10 days before harvest. RB and sunlight were used as the control for artificial and natural light respectively. Plants grown under the UVGR1 had the highest dry leaf biomass accumulation of 4.75 g plant⁻¹ (P < 0.05), 458% and 660% higher than the RB (0.98 g plant⁻¹) and natural sunlight (0.72 g plant⁻¹) controls. UVA had the highest metabolite (Stevioside + Rebaudioside A) concentration of 27% (P < 0.05) compared to the RB and sunlight controls at 17.24% and 15% respectively. The 10 day pre-harvest treatment with blue supplemented light yielded a dry biomass of 1.87 g plant^-1, a 190% increase compared to the RB control. However, the 3 day pre-harvest treatment had higher metabolite yields improvements compared to 10 day treatments with the highest yield obtained of 21.10% in 3-day pre-harvest irradiation that had supplemental UVA and blue light. UVGR1 was the most productive lighting strategy, resulting in the highest overall metabolite yield per plant.

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绿光谱和 UVA 光谱以及收获前处理对室内栽培甜叶菊生物量和代谢物产量的影响

甜叶菊（Stevia rebaudiana）是一种高价值作物，因为其代谢产物（甜菊糖苷，SG）作为一种有机低热量甜味剂，其甜度是传统蔗糖的 300 倍，商业需求旺盛。本研究进行了两项实验。在第一项实验中，使用了不同绿度（GR1 & GR2）、UVA（UV1 & UV2）的处理，以及两者都有的处理（UVGR1、UVGR2）。在第二项实验中，在收获前 3 天和 10 天，分别将一组植物置于红-蓝光（RB）和自然光下，然后再分别转到 GR2、UV2、UVGR2 以及蓝光、绿光和 UVA 的单色光处理中。RB 和阳光分别作为人工光照和自然光照的对照。在 UVGR1 下生长的植物干叶生物量积累最高，为 4.75 g plant-1（P < 0.05），比 RB（0.98 g plant-1）和自然光（0.72 g plant-1）对照分别高 458% 和 660%。UVA 的代谢物（甜菊糖甙 + 甜菊糖甙 A）浓度最高，达到 27% (P < 0.05)，而 RB 和日照对照分别为 17.24% 和 15%。收获前 10 天的蓝光补充处理产生的干生物量为 1.87 g plant-1，比 RB 对照组增加了 190%。然而，与 10 天处理相比，收获前 3 天处理的代谢物产量提高了，收获前 3 天补充 UVA 和蓝光照射的产量最高，达到 21.10%。UVGR1 是产量最高的光照策略，每株植物的总代谢物产量最高。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.