{"title":"Porphyrin modified ZnCo2O4 nanospheres as the excellent peroxidase/oxidase dual nanozymes for colorimetric sensing of cholesterol","authors":"","doi":"10.1016/j.colsurfa.2024.135685","DOIUrl":null,"url":null,"abstract":"<div><div>5,10,15,20-Tetrakis(4-carboxyl phenyl)porphyrin (H<sub>2</sub>TCPP) modified ZnCo<sub>2</sub>O<sub>4</sub> nanospheres (H<sub>2</sub>TCPP-ZnCo<sub>2</sub>O<sub>4</sub>) were prepared by a two-step hydrothermal method. Compared with pure ZnCo<sub>2</sub>O<sub>4</sub>, H<sub>2</sub>TCPP-ZnCo<sub>2</sub>O<sub>4</sub> possessed higher peroxidase-like activity and fast catalyze the chromogenic substrate 3,3,5,5-tetramethylbenzidine (TMB) into oxTMB by H<sub>2</sub>O<sub>2</sub> with a blue change. The catalytic behaviors of H<sub>2</sub>TCPP-ZnCo<sub>2</sub>O<sub>4</sub> is consistent with the Michaelis–Menten equation. Several techniques including electrochemical, fluorescent probes and capture agents were used to investigate the catalytic mechanism, which was identified as electron transfer and superoxide radical (•O<sub>2</sub><sup>−</sup>), due to the synergetic effect between porphyrin molecules and ZnCo<sub>2</sub>O<sub>4</sub>. Based on the nanoperoxidase activity of H<sub>2</sub>TCPP-ZnCo<sub>2</sub>O<sub>4,</sub> a facile colorimetric sensing platforms was designed for the determination of H<sub>2</sub>O<sub>2</sub> and cholesterol. The linear range for H<sub>2</sub>O<sub>2</sub> determination is 100–200 μM, with a detection limit of 76.297 μM as well as cholesterol is 100–900 μM, with a detection limit of 40.027 μM</div></div>","PeriodicalId":278,"journal":{"name":"Colloids and Surfaces A: Physicochemical and Engineering Aspects","volume":null,"pages":null},"PeriodicalIF":4.9000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Colloids and Surfaces A: Physicochemical and Engineering Aspects","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0927775724025494","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
引用次数: 0
Abstract
5,10,15,20-Tetrakis(4-carboxyl phenyl)porphyrin (H2TCPP) modified ZnCo2O4 nanospheres (H2TCPP-ZnCo2O4) were prepared by a two-step hydrothermal method. Compared with pure ZnCo2O4, H2TCPP-ZnCo2O4 possessed higher peroxidase-like activity and fast catalyze the chromogenic substrate 3,3,5,5-tetramethylbenzidine (TMB) into oxTMB by H2O2 with a blue change. The catalytic behaviors of H2TCPP-ZnCo2O4 is consistent with the Michaelis–Menten equation. Several techniques including electrochemical, fluorescent probes and capture agents were used to investigate the catalytic mechanism, which was identified as electron transfer and superoxide radical (•O2−), due to the synergetic effect between porphyrin molecules and ZnCo2O4. Based on the nanoperoxidase activity of H2TCPP-ZnCo2O4, a facile colorimetric sensing platforms was designed for the determination of H2O2 and cholesterol. The linear range for H2O2 determination is 100–200 μM, with a detection limit of 76.297 μM as well as cholesterol is 100–900 μM, with a detection limit of 40.027 μM
期刊介绍:
Colloids and Surfaces A: Physicochemical and Engineering Aspects is an international journal devoted to the science underlying applications of colloids and interfacial phenomena.
The journal aims at publishing high quality research papers featuring new materials or new insights into the role of colloid and interface science in (for example) food, energy, minerals processing, pharmaceuticals or the environment.