Astaxanthin mediated repair of tBHP-Induced cellular injury in chondrocytes.

IF 5.2 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Redox Report Pub Date : 2024-12-01 Epub Date: 2024-11-04 DOI:10.1080/13510002.2024.2422271

Wenwei Liang, Gang Liu, Weibo Zhou, Wei Chen, Yaojun Lu, Hao Wu, Yao Qin, Chunhui Zhu

{"title":"Astaxanthin mediated repair of tBHP-Induced cellular injury in chondrocytes.","authors":"Wenwei Liang, Gang Liu, Weibo Zhou, Wei Chen, Yaojun Lu, Hao Wu, Yao Qin, Chunhui Zhu","doi":"10.1080/13510002.2024.2422271","DOIUrl":null,"url":null,"abstract":"Objective: This study investigates how astaxanthin (AST) counters tert-butyl hydroperoxide (tBHP)-induced cellular damage in C28/I2 chondrocytes, focusing on the circ-HP1BP3/miR-139-5p/SOD1 signaling pathway and its use in sustained-release microspheres for osteoarthritis treatment.Methods: We employed a variety of techniques including real-time quantitative PCR, Western blot, ELISA, and dual-luciferase reporter gene assays to explore AST's molecular effects. Additionally, the efficacy of AST-loaded sustained-release microspheres was evaluated in vitro and in a mouse model of osteoarthritis.Results: AST significantly enhanced SOD1 expression, reducing apoptosis and inflammation in damaged cells. The AST-loaded microspheres showed promising in vitro drug release, improved cell viability, and reduced oxidative stress. In the osteoarthritis mouse model, they effectively decreased joint inflammation and increased the expression of chondrocyte markers.Conclusion: Astaxanthin effectively mitigates oxidative stress and inflammation in chondrocytes via the circ-HP1BP3/miR-139-5p/SOD1 pathway. The development of AST-loaded microspheres offers a novel and promising approach for osteoarthritis therapy, potentially extending to osteoarthritis treatment.","PeriodicalId":21096,"journal":{"name":"Redox Report","volume":"29 1","pages":"2422271"},"PeriodicalIF":5.2000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11536701/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Redox Report","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/13510002.2024.2422271","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/11/4 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: This study investigates how astaxanthin (AST) counters tert-butyl hydroperoxide (tBHP)-induced cellular damage in C28/I2 chondrocytes, focusing on the circ-HP1BP3/miR-139-5p/SOD1 signaling pathway and its use in sustained-release microspheres for osteoarthritis treatment.

Methods: We employed a variety of techniques including real-time quantitative PCR, Western blot, ELISA, and dual-luciferase reporter gene assays to explore AST's molecular effects. Additionally, the efficacy of AST-loaded sustained-release microspheres was evaluated in vitro and in a mouse model of osteoarthritis.

Results: AST significantly enhanced SOD1 expression, reducing apoptosis and inflammation in damaged cells. The AST-loaded microspheres showed promising in vitro drug release, improved cell viability, and reduced oxidative stress. In the osteoarthritis mouse model, they effectively decreased joint inflammation and increased the expression of chondrocyte markers.

Conclusion: Astaxanthin effectively mitigates oxidative stress and inflammation in chondrocytes via the circ-HP1BP3/miR-139-5p/SOD1 pathway. The development of AST-loaded microspheres offers a novel and promising approach for osteoarthritis therapy, potentially extending to osteoarthritis treatment.

查看原文本刊更多论文

虾青素介导的软骨细胞对 tBHP 诱导的细胞损伤的修复。

研究目的本研究探讨虾青素（AST）如何对抗叔丁基过氧化氢（tBHP）诱导的C28/I2软骨细胞的细胞损伤，重点关注circ-HP1BP3/miR-139-5p/SOD1信号通路及其在骨关节炎缓释微球治疗中的应用：我们采用了包括实时定量 PCR、Western 印迹、ELISA 和双荧光素酶报告基因检测在内的多种技术来探讨 AST 的分子效应。此外，还在体外和骨关节炎小鼠模型中评估了AST负载型缓释微球的疗效：结果：AST能明显提高SOD1的表达，减少受损细胞的凋亡和炎症反应。AST 负载微球显示出良好的体外药物释放效果，提高了细胞活力，降低了氧化应激。在骨关节炎小鼠模型中，它们能有效减少关节炎症，增加软骨细胞标志物的表达：结论：虾青素可通过circ-HP1BP3/miR-139-5p/SOD1途径有效缓解软骨细胞的氧化应激和炎症。AST负载微球的开发为骨关节炎的治疗提供了一种新颖而有前景的方法，并有可能扩展到骨关节炎的治疗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Redox Report 生物-生化与分子生物学

CiteScore

6.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Redox Report is a multidisciplinary peer-reviewed open access journal focusing on the role of free radicals, oxidative stress, activated oxygen, perioxidative and redox processes, primarily in the human environment and human pathology. Relevant papers on the animal and plant environment, biology and pathology will also be included. While emphasis is placed upon methodological and intellectual advances underpinned by new data, the journal offers scope for review, hypotheses, critiques and other forms of discussion.