Molecular analysis of vector-borne pathogens in Eurasian badgers (Meles meles) from continental Europe.

IF 3 2区 医学 Q1 PARASITOLOGY
Zoë Tess Lara Lindhorst, Sebastian Brandstetter, Maria Sophia Unterköfler, Barbara Eigner, Joachim Spergser, Marc Colyn, Peter Steinbach, Duško Ćirović, Nikica Šprem, Tomislav Dumić, Vincenzo Veneziano, Franz Müller, Josef Harl, Georgiana Deak, Angela Monica Ionică, Mike Heddergott, Hans-Peter Fuehrer
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引用次数: 0

Abstract

Background: Vector-borne pathogens (VBPs) are increasing in significance in veterinary medicine and public health settings, with wildlife playing a potentially crucial role in their transmission. Eurasian badgers (Meles meles) are widely distributed across Europe. However, information currently available on the prevalence of VBPs in badgers is limited. The objective of the current study was to investigate the occurrence of Anaplasmataceae, Bartonella spp., Mycoplasma spp., Rickettsia spp., Piroplasmida, Trypanosomatida and Filarioidea in badgers and subsequently, based on the results, assess the potential risk to domestic animals, other wildlife and humans.

Methods: Between 2017 and 2021, blood or spleen samples from 220 badgers were collected in nine continental European countries: Austria (n = 7), Bosnia and Herzegovina (n = 2), Croatia (n = 22), France (n = 44), Germany (n = 16), Hungary (n = 7), Italy (n = 16), Romania (n = 80) and Serbia (n = 26). VBPs were identified by performing PCR analysis on the samples, followed by Sanger sequencing. Additionally, to distinguish between different Babesia lineages we performed restriction fragment length polymorphism (RFLP) analysis on piroplasm-positive samples, using HinfI as restriction enzyme. A phylogenetic analysis was performed on Mycoplasma spp.

Results: The pathogens identified were Babesia sp. badger type A (54%), B (23%), and C (37%); Trypanosoma pestanai (56%); Mycoplasma sp. (34%); Candidatus Mycoplasma haematomelis (8%); Candidatus Mycoplasma haematominutum (0.5%); and Ehrlichia spp. (2%). Rickettsia spp., Bartonella spp. and filarioid nematodes were not detected among the tested samples.

Conclusions: The large sample size and diverse study populations in this study provide valuable insights into the distribution and epidemiology of the analyzed pathogens. Some of the VBPs identified in our study show high similarity to those found in domestic animals, such as dogs. This finding suggests that badgers, as potential reservoirs for these pathogens, may pose a threat not only to other wildlife but also to domestic animals in close vicinity. Continuous surveillance is essential to monitor VBPs in wildlife as a means to enable the assessment of their impact on other wildlife species, domestic animals and human health.

欧洲大陆欧亚獾(Meles meles)中病媒传播病原体的分子分析。
背景:病媒传播的病原体(VBPs)在兽医和公共卫生领域的重要性与日俱增,而野生动物在其传播过程中可能扮演着至关重要的角色。欧亚獾(Meles meles)广泛分布于欧洲。然而,目前有关獾中 VBPs 流行情况的信息非常有限。本研究的目的是调查獾中无鞭毛虫科、巴顿氏菌属、支原体属、立克次体属、螺旋体属、锥虫属和丝虫属的发生情况,并根据结果评估其对家畜、其他野生动物和人类的潜在风险:方法:2017 年至 2021 年期间,在九个欧洲大陆国家收集了 220 只獾的血液或脾脏样本:奥地利(n = 7)、波斯尼亚和黑塞哥维那(n = 2)、克罗地亚(n = 22)、法国(n = 44)、德国(n = 16)、匈牙利(n = 7)、意大利(n = 16)、罗马尼亚(n = 80)和塞尔维亚(n = 26)。通过对样本进行 PCR 分析,然后进行 Sanger 测序,确定了 VBPs。此外,为了区分不同的巴贝西亚菌系,我们使用 HinfI 作为限制性酶,对 piroplasm 阳性样本进行了限制性片段长度多态性 (RFLP) 分析。对支原体属进行了系统进化分析:鉴定出的病原体有:獾巴贝斯虫 A 型(54%)、B 型(23%)和 C 型(37%);鼠疫锥虫(56%);支原体(34%);血型支原体(8%);血型支原体(0.5%);埃立克次氏体(2%)。检测样本中未发现立克次体属、巴顿氏菌属和丝状线虫:这项研究的样本量大、研究人群多样,为分析病原体的分布和流行病学提供了宝贵的见解。在我们的研究中发现的一些 VBPs 与在狗等家畜身上发现的 VBPs 高度相似。这一发现表明,獾作为这些病原体的潜在储库,不仅可能对其他野生动物构成威胁,也可能对附近的家畜构成威胁。持续监测对监测野生动物中的吠形吠声病原体至关重要,这是评估这些病原体对其他野生动物物种、家养动物和人类健康的影响的一种手段。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Parasites & Vectors
Parasites & Vectors 医学-寄生虫学
CiteScore
6.30
自引率
9.40%
发文量
433
审稿时长
1.4 months
期刊介绍: Parasites & Vectors is an open access, peer-reviewed online journal dealing with the biology of parasites, parasitic diseases, intermediate hosts, vectors and vector-borne pathogens. Manuscripts published in this journal will be available to all worldwide, with no barriers to access, immediately following acceptance. However, authors retain the copyright of their material and may use it, or distribute it, as they wish. Manuscripts on all aspects of the basic and applied biology of parasites, intermediate hosts, vectors and vector-borne pathogens will be considered. In addition to the traditional and well-established areas of science in these fields, we also aim to provide a vehicle for publication of the rapidly developing resources and technology in parasite, intermediate host and vector genomics and their impacts on biological research. We are able to publish large datasets and extensive results, frequently associated with genomic and post-genomic technologies, which are not readily accommodated in traditional journals. Manuscripts addressing broader issues, for example economics, social sciences and global climate change in relation to parasites, vectors and disease control, are also welcomed.
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