Allograft to bone-tunnel integration in a canine anterior cruciate ligament reconstruction model: a comparison study of allograft preparation methods.

IF 2.8 3区 医学 Q1 ORTHOPEDICS
Xiaoke Shang, Jianzhong Hu, Jin Qu, Peng Wen, Jian Li, Qi Li, Jun Zheng
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Abstract

The procedure of anterior cruciate ligament (ACL) allograft preparation can be divided into fresh-frozen method (FF-allograft) or freeze-dried method (FD-allograft). This study aims to biomechanically and histologically compare the graft to bone tunnel integration between the two allografts. In-vitro results indicated that FF-allograft and FD-allograft showed excellent biocompatibility and biomechanics, while FD-allograft showed a denser collagen fiber arrangement than FF-allograft and autograft. Then, in-vivo preformation of the FF-allograft, FD-allograft, and autograft on bone tunnel integration was evaluated via a canine ACL reconstruction model. In-vivo results indicated that no signs of infection or osteoarthritis were shown in the femur-graft-tibia complexes, but more vascularity and synovitis formed around the implanted FF-allograft. Micro-computed tomography showed that peri-graft bone in the FF-allograft group was significantly increased and remodeled compared with the FD-allograft group; Histologically, the FF-allograft group exhibited similar graft-bone tunnel healing to the FD-allograft group. Tartrate-resistant acid phosphatase (TRAP) staining showed significantly more osteoclasts presented in the FD-allograft group compared to the FF-allograft group. Meanwhile, a significantly higher failure load was shown in the FF-allograft group when compared with the FD-allograft group (P < 0.05). In conclusion, the FF-allograft integrated more firmly into the bone tunnel than the FD-allograft when used in ACL reconstruction.

犬前十字韧带重建模型中同种异体移植物与骨隧道的整合:同种异体移植物制备方法的比较研究。
前交叉韧带(ACL)异体移植物的制备方法可分为新鲜冷冻法(FF-allograft)和冷冻干燥法(FD-allograft)。本研究旨在从生物力学和组织学角度比较两种异体移植物与骨隧道的整合情况。体外实验结果表明,冻干异体移植物和冻干异体移植物具有良好的生物相容性和生物力学特性,而冻干异体移植物的胶原纤维排列密度高于冻干异体移植物和自体移植物。然后,通过犬前交叉韧带重建模型评估了 FF-同种异体移植物、FD-同种异体移植物和自体移植物的体内预成形对骨隧道整合的影响。体内结果显示,股骨-移植物-胫骨复合体未出现感染或骨关节炎迹象,但植入的 FF-异体移植物周围形成了更多的血管和滑膜炎。显微计算机断层扫描显示,与 FD-allograft 组相比,FF-allograft 组的移植物周围骨明显增加和重塑;组织学上,FF-allograft 组与 FD-allograft 组表现出相似的移植物-骨隧道愈合。耐酒石酸磷酸酶(TRAP)染色显示,FD-异体移植组的破骨细胞明显多于 FF-异体移植组。同时,与 FD-同种异体移植组相比,FF-同种异体移植组的失败载荷明显更高(P<0.05)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
4.10
自引率
7.70%
发文量
494
审稿时长
>12 weeks
期刊介绍: Journal of Orthopaedic Surgery and Research is an open access journal that encompasses all aspects of clinical and basic research studies related to musculoskeletal issues. Orthopaedic research is conducted at clinical and basic science levels. With the advancement of new technologies and the increasing expectation and demand from doctors and patients, we are witnessing an enormous growth in clinical orthopaedic research, particularly in the fields of traumatology, spinal surgery, joint replacement, sports medicine, musculoskeletal tumour management, hand microsurgery, foot and ankle surgery, paediatric orthopaedic, and orthopaedic rehabilitation. The involvement of basic science ranges from molecular, cellular, structural and functional perspectives to tissue engineering, gait analysis, automation and robotic surgery. Implant and biomaterial designs are new disciplines that complement clinical applications. JOSR encourages the publication of multidisciplinary research with collaboration amongst clinicians and scientists from different disciplines, which will be the trend in the coming decades.
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