A novel protein elicitor (Cs08297) from Ciboria shiraiana enhances plant disease resistance.

IF 4.8 1区 农林科学 Q1 PLANT SCIENCES
Shuai Zhang, Ruolan Li, Wei Fan, Xuefei Chen, Caiquan Tao, Shuman Liu, Panpan Zhu, Shuchang Wang, Aichun Zhao
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Abstract

Ciboria shiraiana is a necrotrophic fungus that causes mulberry sclerotinia disease resulting in huge economic losses in agriculture. During infection, the fungus uses immunity elicitors to induce plant tissue necrosis that could facilitate its colonization on plants. However, the key elicitors and immune mechanisms remain unclear in C. shiraiana. Herein, a novel elicitor Cs08297 secreted by C. shiraiana was identified, and it was found to target the apoplast in plants to induce cell death. Cs08297 is a cysteine-rich protein unique to C. shiraiana, and cysteine residues in Cs08297 were crucial for its ability to induce cell death. Cs08297 induced a series of defence responses in Nicotiana benthamiana, including the burst of reactive oxygen species (ROS), callose deposition, and activation of defence-related genes. Cs08297 induced-cell death was mediated by leucine-rich repeat (LRR) receptor-like kinases BAK1 and SOBIR1. Purified His-tagged Cs08297-thioredoxin fusion protein triggered cell death in different plants and enhanced plant resistance to diseases. Cs08297 was necessary for sclerotial development, oxidative-stress adaptation, and cell wall integrity but negatively regulated virulence of C. shiraiana. In conclusion, our results revealed that Cs08297 is a novel fungal elicitor in fungi inducing plant immunity. Furthermore, its potential to enhance plant resistance provides a new target to control agricultural diseases biologically.

一种来自 Ciboria shiraiana 的新型蛋白诱导剂(Cs08297)可增强植物的抗病性。
Ciboria shiraiana 是一种坏死性真菌,会引起桑硬皮病,给农业造成巨大的经济损失。在感染过程中,真菌利用免疫诱导物诱导植物组织坏死,从而促进其在植物上的定殖。然而,C. shiraiana 的关键诱导物和免疫机制仍不清楚。在本文中,研究人员发现了一种由 C. shiraiana 分泌的新型诱导剂 Cs08297,该诱导剂可靶向植物的凋亡体诱导细胞死亡。Cs08297是C. shiraiana特有的富含半胱氨酸的蛋白质,Cs08297中的半胱氨酸残基对其诱导细胞死亡的能力至关重要。Cs08297 能诱导烟草中的一系列防御反应,包括活性氧(ROS)猝灭、胼胝质沉积和防御相关基因的激活。Cs08297 诱导的细胞死亡是由富含亮氨酸重复(LRR)的受体样激酶 BAK1 和 SOBIR1 介导的。纯化的 His-tagged Cs08297-硫氧化还蛋白融合蛋白可引发不同植物的细胞死亡,并增强植物的抗病性。Cs08297 是硬壳菌发育、氧化应激适应和细胞壁完整性所必需的,但对 C. shiraiana 的毒力有负向调节作用。总之,我们的研究结果表明,Cs08297 是真菌诱导植物免疫的一种新型真菌诱导物。此外,其增强植物抗性的潜力为农业病害的生物防治提供了一个新的目标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Molecular plant pathology
Molecular plant pathology 生物-植物科学
CiteScore
9.40
自引率
4.10%
发文量
120
审稿时长
6-12 weeks
期刊介绍: Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
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