Effects of Toothpaste Containing 2% Zinc Citrate on Gingival Health and Three Related Bacteria—A Randomized Double-Blind Study

IF 1.7 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research Pub Date : 2024-11-04 DOI:10.1002/cre2.70020

Yujie Zhou, Yi Zhou, Binyou Liao, Xiaobin Chen, Yulong Niu, Biao Ren

{"title":"Effects of Toothpaste Containing 2% Zinc Citrate on Gingival Health and Three Related Bacteria—A Randomized Double-Blind Study","authors":"Yujie Zhou, Yi Zhou, Binyou Liao, Xiaobin Chen, Yulong Niu, Biao Ren","doi":"10.1002/cre2.70020","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objectives</h3>\n \n <p>Gingivitis is the initial stage of periodontitis, one of the most common oral diseases and the primary cause of tooth loss. This study aims to evaluate the effect of toothpaste containing 2% zinc citrate on gingival health and the abundance of three bacteria related to gingivitis and periodontitis.</p>\n </section>\n \n <section>\n \n <h3> Methods and Materials</h3>\n \n <p>Eleven volunteers with the same oral health status were randomly assigned to the treatment (<i>n</i> = 5) and control (<i>n</i> = 6) groups. The control group used fluoride toothpaste, while the treatment group used fluoride toothpaste supplemented with 2% zinc citrate for 3 months. The plaque index, gingival index, and bleeding index were measured at baseline (0 day), 3 weeks, and 3 months. Dental plaque from four areas of the mouth (FDI criteria) was collected at the same timepoints. A total of 132 dental plaque samples were analyzed using quantitative PCR (qPCR) to monitor the abundance of <i>Actinobacillus actinomycetemcomitans</i>, <i>Porphyromonas gingivalis</i>, and <i>Tannerella forsythia</i>.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Toothpaste containing 2% zinc citrate significantly lowered the gingival index and reduced gum bleeding but did not affect the plaque index. It also reduced the total abundance of the three bacteria related to gingivitis and periodontitis in dental plaque over a long-term period.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>Toothpaste with 2% zinc citrate persistently improves gingival health and reduces the presence of gingivitis-associated bacteria in dental plaque.</p>\n </section>\n \n <section>\n \n <h3> Trial Registration</h3>\n \n <p>Chinese Clinical Trial Registry (Clinical trial registration no.: ChiCTR1900020592) (09/01/2019).</p>\n </section>\n </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70020","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical and Experimental Dental Research","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cre2.70020","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives

Gingivitis is the initial stage of periodontitis, one of the most common oral diseases and the primary cause of tooth loss. This study aims to evaluate the effect of toothpaste containing 2% zinc citrate on gingival health and the abundance of three bacteria related to gingivitis and periodontitis.

Methods and Materials

Eleven volunteers with the same oral health status were randomly assigned to the treatment (n = 5) and control (n = 6) groups. The control group used fluoride toothpaste, while the treatment group used fluoride toothpaste supplemented with 2% zinc citrate for 3 months. The plaque index, gingival index, and bleeding index were measured at baseline (0 day), 3 weeks, and 3 months. Dental plaque from four areas of the mouth (FDI criteria) was collected at the same timepoints. A total of 132 dental plaque samples were analyzed using quantitative PCR (qPCR) to monitor the abundance of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Tannerella forsythia.

Results

Toothpaste containing 2% zinc citrate significantly lowered the gingival index and reduced gum bleeding but did not affect the plaque index. It also reduced the total abundance of the three bacteria related to gingivitis and periodontitis in dental plaque over a long-term period.

Conclusions

Toothpaste with 2% zinc citrate persistently improves gingival health and reduces the presence of gingivitis-associated bacteria in dental plaque.

Trial Registration

Chinese Clinical Trial Registry (Clinical trial registration no.: ChiCTR1900020592) (09/01/2019).

Abstract Image

查看原文本刊更多论文

含 2% 柠檬酸锌的牙膏对牙龈健康和三种相关细菌的影响--随机双盲研究

目的：牙龈炎是牙周炎的初期阶段，牙周炎是最常见的口腔疾病之一，也是导致牙齿脱落的主要原因。本研究旨在评估含 2% 柠檬酸锌的牙膏对牙龈健康以及与牙龈炎和牙周炎有关的三种细菌数量的影响：将 11 名口腔健康状况相同的志愿者随机分配到治疗组（5 人）和对照组（6 人）。对照组使用含氟牙膏，治疗组使用含氟牙膏并添加 2% 柠檬酸锌，为期 3 个月。分别在基线（0 天）、3 周和 3 个月时测量牙菌斑指数、牙龈指数和出血指数。在相同的时间点收集口腔四个部位的牙菌斑（FDI 标准）。共对 132 份牙菌斑样本进行了定量 PCR（qPCR）分析，以监测放线杆菌、牙龈卟啉单胞菌和连翘丹那菌的数量：结果：含 2% 柠檬酸锌的牙膏能明显降低牙龈指数，减少牙龈出血，但不影响牙菌斑指数。它还能长期降低牙菌斑中与牙龈炎和牙周炎有关的三种细菌的总含量：结论：含2%柠檬酸锌的牙膏可持续改善牙龈健康，减少牙菌斑中牙龈炎相关细菌的数量：中国临床试验注册中心（临床试验注册号：ChiCTR1900020592）（09/01/2019）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Clinical and Experimental Dental Research DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.30

自引率

5.60%

发文量

165

审稿时长

26 weeks

期刊介绍： Clinical and Experimental Dental Research aims to provide open access peer-reviewed publications of high scientific quality representing original clinical, diagnostic or experimental work within all disciplines and fields of oral medicine and dentistry. The scope of Clinical and Experimental Dental Research comprises original research material on the anatomy, physiology and pathology of oro-facial, oro-pharyngeal and maxillofacial tissues, and functions and dysfunctions within the stomatognathic system, and the epidemiology, aetiology, prevention, diagnosis, prognosis and therapy of diseases and conditions that have an effect on the homeostasis of the mouth, jaws, and closely associated structures, as well as the healing and regeneration and the clinical aspects of replacement of hard and soft tissues with biomaterials, and the rehabilitation of stomatognathic functions. Studies that bring new knowledge on how to advance health on the individual or public health levels, including interactions between oral and general health and ill-health are welcome.