Superior target genes and pathways for RNAi-mediated pest control revealed by genome-wide analysis in the beetle Tribolium castaneum.

IF 3.8 1区农林科学 Q1 AGRONOMY

Pest Management Science Pub Date : 2024-11-05 DOI:10.1002/ps.8505

Benjamin Buer, Jürgen Dönitz, Martin Milner, Sonja Mehlhorn, Claudia Hinners, Janna Siemanowski-Hrach, Julia K Ulrich, Daniela Großmann, Doga Cedden, Ralf Nauen, Sven Geibel, Gregor Bucher

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引用次数: 0

Abstract

Background: An increasing human population, the emergence of resistances against pesticides and their potential impact on the environment call for the development of new eco-friendly pest control strategies. RNA interference (RNAi)-based pesticides have emerged as a new option with the first products entering the market. Essentially, double-stranded RNAs targeting essential genes of pests are either expressed in the plants or sprayed on their surface. Upon feeding, pests mount an RNAi response and die. However, it has remained unclear whether RNAi-based insecticides should target the same pathways as classic pesticides or whether the different mode-of-action would favor other processes. Moreover, there is no consensus on the best genes to be targeted.

Results: We performed a genome-wide screen in the red flour beetle to identify 905 RNAi target genes. Based on a validation screen and clustering, we identified the 192 most effective target genes in that species. The transfer to oral application in other beetle pests revealed a list of 34 superior target genes, which are an excellent starting point for application in other pests. Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) analyses of our genome-wide dataset revealed that genes with high efficacy belonged mainly to basic cellular processes such as gene expression and protein homeostasis - processes not targeted by classic insecticides.

Conclusion: Our work revealed the best target genes and target processes for RNAi-based pest control and we propose a procedure to transfer our short list of superior target genes to other pests. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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通过对甲虫Tribolium castaneum的全基因组分析，发现RNAi介导的害虫控制的优良目标基因和途径。

背景：随着人口的不断增长、杀虫剂抗药性的出现以及杀虫剂对环境的潜在影响，人们需要开发新的生态友好型害虫控制策略。随着首批产品进入市场，基于 RNA 干扰（RNAi）的杀虫剂已成为一种新的选择。从本质上讲，针对害虫重要基因的双链 RNA 要么在植物体内表达，要么喷洒在植物表面。害虫在摄食后会产生 RNAi 反应并死亡。然而，基于 RNAi 的杀虫剂是否应针对与传统杀虫剂相同的途径，或者不同的作用模式是否会有利于其他过程，目前仍不清楚。此外，对于最佳靶向基因也没有达成共识：结果：我们对红粉甲虫进行了全基因组筛选，确定了 905 个 RNAi 靶基因。根据验证筛选和聚类，我们确定了该物种中 192 个最有效的靶基因。将其转移到其他甲虫害虫的口服应用中，发现了 34 个优质靶基因，这是在其他害虫中应用的良好起点。对我们的全基因组数据集进行的基因本体（GO）和京都基因和基因组百科全书（KEGG）分析表明，高效基因主要属于基本细胞过程，如基因表达和蛋白质稳态--传统杀虫剂不针对这些过程：我们的工作揭示了基于 RNAi 的害虫控制的最佳靶基因和靶过程，我们还提出了一个将我们的优秀靶基因短名单应用于其他害虫的程序。© 2024 作者简介害虫管理科学》由 John Wiley & Sons Ltd 代表化学工业协会出版。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.