Neurodevelopmental Process Monitoring of Cytosine Arabinoside-Exposed Neurons Using Raman Spectroscopy.

IF 2.2 3区 化学 Q2 INSTRUMENTS & INSTRUMENTATION
Kosuke Hashimoto, Hidetoshi Sato
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Abstract

Raman spectroscopy is used to monitor the development of live neurons exposed to cytosine arabinoside (ara-C). Ara-C is widely used to culture neurons and exclude non-neuronal cells. In this study, Raman spectra obtained from neurons exposed to ara-C were plotted using an analytical model of neuronal development to evaluate the impact of ara-C on neuronal development. After two days of culturing, neurons were exposed to ara-C for 24 h at final concentrations of 0 (control), 5, and 25 μM. Principal component analysis (PCA) was performed to build an analytical model for evaluating neurodevelopmental disorders caused by ara-C treatment. We projected the Raman spectra obtained from ara-C-treated cells onto the control group dataset. The distribution of PC1 scores for neurons exposed to ara-C at a final concentration of 5 μM was not significantly different from that of the control group. In contrast, under a final concentration of 25 μM, the data population at 10 and 15 days of culturing overlapped significantly with that of neurons at 4 days of normal culturing. These results suggest that Raman spectroscopy can detect very small physiological alterations in the neurons even after a short-term exposure (24 h) of ara-C. Our analytical method has high potential to evaluate the developmental stages for living neurons under exposure to chemicals.

利用拉曼光谱监测暴露于阿拉伯苷的胞嘧啶神经元的神经发育过程
拉曼光谱用于监测暴露于阿糖胞苷(Ara-C)的活神经元的发育情况。Ara-C 被广泛用于培养神经元和排除非神经元细胞。本研究利用神经元发育分析模型绘制了暴露于 Ara-C 的神经元的拉曼光谱,以评估 Ara-C 对神经元发育的影响。培养两天后,将神经元暴露于最终浓度为 0(对照组)、5 和 25 μM 的 ara-C 中 24 小时。我们进行了主成分分析(PCA),以建立一个分析模型,用于评估阿拉卡治疗导致的神经发育障碍。我们将从阿拉卡处理过的细胞中获得的拉曼光谱投射到对照组数据集上。最终浓度为 5 μM 的阿拉卡暴露神经元的 PC1 得分分布与对照组无显著差异。相反,在最终浓度为 25 μM 时,培养 10 天和 15 天的数据群与正常培养 4 天的神经元数据群明显重叠。这些结果表明,即使在短期(24 小时)接触 ara-C 后,拉曼光谱也能检测到神经元中非常微小的生理变化。我们的分析方法在评估暴露于化学物质的活体神经元的发育阶段方面具有很大的潜力。
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来源期刊
Applied Spectroscopy
Applied Spectroscopy 工程技术-光谱学
CiteScore
6.60
自引率
5.70%
发文量
139
审稿时长
3.5 months
期刊介绍: Applied Spectroscopy is one of the world''s leading spectroscopy journals, publishing high-quality peer-reviewed articles, both fundamental and applied, covering all aspects of spectroscopy. Established in 1951, the journal is owned by the Society for Applied Spectroscopy and is published monthly. The journal is dedicated to fulfilling the mission of the Society to “…advance and disseminate knowledge and information concerning the art and science of spectroscopy and other allied sciences.”
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