A plastidial lipoyl synthase LIP1p plays a crucial role in phosphate homeostasis in Arabidopsis

IF 6.2 1区 生物学 Q1 PLANT SCIENCES
Shenghong Ge, Xinlong Xiao, Ke Zhang, Changhong Yang, Jinsong Dong, Keying Chen, Qiuyu Lv, Viswanathan Satheesh, Mingguang Lei
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Abstract

Phosphate (Pi) homeostasis is important for plant growth and adaptation to the dynamic environment, which requires the precise regulation of phosphate transporter (PHT) trafficking from the endoplasmic reticulum to the plasma membrane. LIPOYL SYNTHASE 1p (LIP1p) is known as a key enzyme in plastids to catalyze lipoylation of pyruvate dehydrogenase complex for de novo fatty acid synthesis. It is unknown whether this process is involved in regulating Pi homeostasis. Here, we demonstrate a new role of LIP1p in controlling Pi homeostasis by regulating PHT1 trafficking. We recovered a weak mutant allele of LIP1p in Arabidopsis that accumulates much less Pi and has enhanced expression of phosphate starvation-induced genes. LIP1p mutation alters the lipid profile and compromises vesicle trafficking of PHT1 to the plasma membrane to impair Pi uptake. Beside phosphorus, the homeostasis of a series of mineral nutrients was also perturbed in lip1p mutant. Our findings provide powerful genetic evidence to support the linkage between lipoylation and ion homeostasis in plants.

拟南芥质体脂酰合成酶 LIP1p 在磷酸盐平衡中发挥着至关重要的作用。
磷酸盐(Pi)平衡对植物生长和适应动态环境非常重要,这需要精确调节磷酸盐转运体(PHT)从内质网到质膜的运输。众所周知,脂酰合成酶 1p(LIP1p)是质体中催化丙酮酸脱氢酶复合物脂酰化以进行新脂肪酸合成的关键酶。目前还不清楚这一过程是否参与调节 Pi 稳态。在这里,我们证明了 LIP1p 通过调节 PHT1 的运输在控制 Pi 稳态中的新作用。我们在拟南芥中发现了一个 LIP1p 的弱突变等位基因,它积累的 Pi 更少,而且磷酸盐饥饿诱导基因的表达增强。LIP1p突变改变了脂质结构,损害了PHT1向质膜的囊泡运输,从而影响了π的吸收。除了磷之外,lip1p突变体中一系列矿质营养物质的平衡也受到了干扰。我们的研究结果为支持植物中脂酰化与离子平衡之间的联系提供了有力的遗传证据。
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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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