HcCYP6AE178 plays a crucial role in facilitating Hyphantria cunea's adaptation to a diverse range of host plants

IF 4.2 1区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
{"title":"HcCYP6AE178 plays a crucial role in facilitating Hyphantria cunea's adaptation to a diverse range of host plants","authors":"","doi":"10.1016/j.pestbp.2024.106194","DOIUrl":null,"url":null,"abstract":"<div><div>Strong multi-host adaptability significantly contributes to the rapid dissemination of <em>Hyphantria cunea.</em> The present study explores the involvement of cytochrome P450 monooxygenase (P450) in the multi-host adaptation of <em>H. cunea</em> and aims to develop RNA pesticides targeting essential P450 genes to disrupt this adaptability. The results showed that inhibiting P450 activity notably reduced larval weight and food-intake across seven plants groups. The P450 gene <em>HcCYP6AE178</em> was highly upregulated in <em>H. cunea</em> larvae from medium- and low-preference host plant groups. Silencing <em>HcCYP6AE178</em> significantly decreased <em>H. cunea</em> larval body weight, increased larval mortality, inhibited energy metabolism genes expression and interfered with growth regulatory genes expression. Overexpression of <em>HcCYP6AE178</em> enhanced the tolerance of <em>Drosophila</em> and Sf9 cells to the plant defensive substances cytisine and coumarin. The RNA pesticide CS-ds<em>HcCYP6AE178</em> constructed using chitosan (CS) exhibited remarkable stability. Treatment with CS-ds<em>HcCYP6AE178</em> effectively reduced <em>H. cunea</em> larval body weight, heightened larval mortality, and disrupted growth regulatory genes expression in low-preference host plant groups. Combined treatment of CS-ds<em>HcCYP6AE178</em> and coumarin significantly elevated <em>H. cunea</em> larval mortality compared to coumarin alone, accompanied by the inhibition of growth regulatory genes expression and an abnormal increase in energy metabolism genes expression. Taken together, <em>HcCYP6AE178</em> is essential for the adaptation of <em>H. cunea</em> to multiple host plants, and RNA pesticides targeting <em>HcCYP6AE178</em> can effectively impair the performance of <em>H. cunea</em> in different host plants.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":null,"pages":null},"PeriodicalIF":4.2000,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pesticide Biochemistry and Physiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0048357524004279","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Strong multi-host adaptability significantly contributes to the rapid dissemination of Hyphantria cunea. The present study explores the involvement of cytochrome P450 monooxygenase (P450) in the multi-host adaptation of H. cunea and aims to develop RNA pesticides targeting essential P450 genes to disrupt this adaptability. The results showed that inhibiting P450 activity notably reduced larval weight and food-intake across seven plants groups. The P450 gene HcCYP6AE178 was highly upregulated in H. cunea larvae from medium- and low-preference host plant groups. Silencing HcCYP6AE178 significantly decreased H. cunea larval body weight, increased larval mortality, inhibited energy metabolism genes expression and interfered with growth regulatory genes expression. Overexpression of HcCYP6AE178 enhanced the tolerance of Drosophila and Sf9 cells to the plant defensive substances cytisine and coumarin. The RNA pesticide CS-dsHcCYP6AE178 constructed using chitosan (CS) exhibited remarkable stability. Treatment with CS-dsHcCYP6AE178 effectively reduced H. cunea larval body weight, heightened larval mortality, and disrupted growth regulatory genes expression in low-preference host plant groups. Combined treatment of CS-dsHcCYP6AE178 and coumarin significantly elevated H. cunea larval mortality compared to coumarin alone, accompanied by the inhibition of growth regulatory genes expression and an abnormal increase in energy metabolism genes expression. Taken together, HcCYP6AE178 is essential for the adaptation of H. cunea to multiple host plants, and RNA pesticides targeting HcCYP6AE178 can effectively impair the performance of H. cunea in different host plants.

Abstract Image

HcCYP6AE178 在促进楔叶象甲适应多种寄主植物方面发挥着关键作用
强烈的多宿主适应性是楔尾象甲迅速传播的重要原因。本研究探讨了细胞色素 P450 单加氧酶(P450)在楔尾蝇多寄主适应性中的参与作用,并旨在开发针对重要 P450 基因的 RNA 杀虫剂来破坏这种适应性。结果表明,抑制 P450 活性可显著降低七个植物组的幼虫体重和摄食量。P450基因HcCYP6AE178在中偏好和低偏好寄主植物组的楔尾蝇幼虫体内高度上调。沉默 HcCYP6AE178 能显著降低楔尾蝇幼虫体重,增加幼虫死亡率,抑制能量代谢基因的表达,干扰生长调节基因的表达。过表达 HcCYP6AE178 能增强果蝇和 Sf9 细胞对植物防御物质胞嘧啶和香豆素的耐受性。利用壳聚糖(CS)构建的 RNA 杀虫剂 CS-dsHcCYP6AE178 具有显著的稳定性。CS-dsHcCYP6AE178能有效降低胭脂虫幼虫体重,增加幼虫死亡率,并干扰低偏好寄主植物群中生长调节基因的表达。与单独使用香豆素相比,CS-dsHcCYP6AE178 和香豆素联合处理可显著提高胭脂虫幼虫的死亡率,同时抑制生长调节基因的表达,并使能量代谢基因的表达异常增加。综上所述,HcCYP6AE178对于楔尾蝇蛆适应多种寄主植物至关重要,以HcCYP6AE178为靶标的RNA杀虫剂可以有效地损害楔尾蝇蛆在不同寄主植物中的表现。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
7.00
自引率
8.50%
发文量
238
审稿时长
4.2 months
期刊介绍: Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance. Research Areas Emphasized Include the Biochemistry and Physiology of: • Comparative toxicity • Mode of action • Pathophysiology • Plant growth regulators • Resistance • Other effects of pesticides on both parasites and hosts.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信