Effects of Laurus nobilis essential oil nano-particles on growth performance, antioxidant and immune responses to bacterial infection in Nile tilapia, Oreochromis niloticus

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture Pub Date : 2024-10-29 DOI:10.1016/j.aquaculture.2024.741821

Morteza Yousefi , Hossein Adineh , Seyyed Morteza Hoseini , Seyed Amir Mehdi Hashemianfar , Evgeny Vladimirovich Kulikov , Nikolay Vladimirovich Petukhov , Tatyana Aleksandrovna Ryzhova

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引用次数: 0

Abstract

This study was designed to assess the effects of four feeds containing 0 (CTL), 25 mg/kg (LN25), 50 mg/kg (LN50) and 100 mg/kg (LN100) Laurus nobilis (LN) essential oil nano-liposomes (LN-NP) on Nile tilapia, Oreochromis niloticus. After 56 days of feeding with the above diets, growth performance and digestive enzyme activities determined in all treatments. Then, the fish were experimentally infected by Aeromonas hydrophila and their survival, antioxidant capacity, biochemical parameters and immune-related transcripts were measured, before and after the infection. The results showed that the growth rate and intestinal activities of amylase, lipase and protease were significantly higher in the LN50 and LN100 treatments than CTL. Post-infection survivals in the LN50 and LN100 treatments were similar and significantly higher than the CTL and LN25 treatments. Compared to the CTL treatment, the LN50 and LN100 treatments showed improved hepatic antioxidant parameters and lower malondialdehyde levels before and/or after infection. Infection significantly decreased plasma lysozyme and complement activities, but the LN50 and LN100 treatments had significantly higher activities before and after infection. These treatments had lower alanine aminotransferase and aspartate aminotransferase activities in plasma before and after infection, compared to the CTL treatment. The expression of tumor necrosis factor-alpha, interleukin-8 and interleukin-1 beta increased significantly in the LN50 and LN100 treatments, before and after infection, when compared to the CTL treatment. Based on the results, LN-NP at a dosage of 50 mg/kg is suitable for feeding Nile tilapia as it is a growth promoter and immunostimulator and increases the resistance of fish to A. hydrophila.

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纳米月桂精油颗粒对尼罗罗非鱼生长性能、抗氧化和细菌感染免疫反应的影响

本研究旨在评估含有 0（CTL）、25 毫克/千克（LN25）、50 毫克/千克（LN50）和 100 毫克/千克（LN100）月桂精油纳米脂质体（LN-NP）的四种饲料对尼罗罗非鱼（Oreochromis niloticus）的影响。投喂上述日粮 56 天后，测定所有处理的生长性能和消化酶活性。然后，用嗜水气单胞菌对鱼类进行实验性感染，并测定感染前后鱼类的存活率、抗氧化能力、生化指标和免疫相关转录物。结果表明，LN50 和 LN100 处理的淀粉酶、脂肪酶和蛋白酶的生长率和肠道活性明显高于 CTL。LN50和LN100处理的感染后存活率相似，明显高于CTL和LN25处理。与 CTL 处理相比，LN50 和 LN100 处理在感染前和/或感染后改善了肝脏抗氧化参数，降低了丙二醛水平。感染明显降低了血浆溶菌酶和补体活性，但LN50和LN100处理在感染前后的溶菌酶和补体活性明显更高。与 CTL 处理相比，这些处理在感染前后血浆中的丙氨酸氨基转移酶和天冬氨酸氨基转移酶活性较低。与 CTL 处理相比，LN50 和 LN100 处理在感染前后的肿瘤坏死因子-α、白细胞介素-8 和白细胞介素-1 beta 的表达量均显著增加。根据研究结果，50 毫克/千克剂量的 LN-NP 适合用于饲喂尼罗罗非鱼，因为它是一种生长促进剂和免疫刺激剂，能增强鱼类对嗜水鲱的抵抗力。

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来源期刊

Aquaculture 农林科学-海洋与淡水生物学

CiteScore

8.60

自引率

17.80%

发文量

1246

审稿时长

56 days

期刊介绍： Aquaculture is an international journal for the exploration, improvement and management of all freshwater and marine food resources. It publishes novel and innovative research of world-wide interest on farming of aquatic organisms, which includes finfish, mollusks, crustaceans and aquatic plants for human consumption. Research on ornamentals is not a focus of the Journal. Aquaculture only publishes papers with a clear relevance to improving aquaculture practices or a potential application.