Fabrication and characterization of Carbon Dot-induced Fe MOF for the detection and destruction of cancer cell

IF 5.45 Q1 Physics and Astronomy

Nano-Structures & Nano-Objects Pub Date : 2024-11-02 DOI:10.1016/j.nanoso.2024.101401

Sayani Maiti , Suvendu Nandi , Swarup Krishna Bhattacharyya , Aparajita Pal , Baidyanath Roy , Samit Kumar Ray , Mahitosh Mandal , Narayan Ch. Das , Susanta Banerjee

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引用次数: 0

Abstract

Developing an effective drug delivery system that targets cancer cells while minimizing the side effects of chemotherapy continues to be a significant challenge in the field of cancer therapy. This work introduces a bio-degradable metal organic framework (MOF) scaffold system that is enhanced with carbon dots (CDs) to facilitate the precise delivery of drugs and tracing of their pathways within cancer cells. We synthesized and reduced an iron based MOF (rMOF) to enable Fenton reactions in cancer cells. The carbon dots derived from folic acid were crosslinked with the rMOF using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide (EDC-NHS) crosslinking chemistry to provide bio-sensing capabilities and subsequent incorporation of the anticancer drug 5 fluorouracil (5 FU) into this system for targeted anticancer activity. The rMOF-CD linkage is disrupted in acidic environments because of amine group protonation, which results in disintegration of the MOF structure and release of Fe²⁺ ions. These ions are capable of destroying cancer cells by engaging in Fenton reactions with the presence of H₂O₂ in the cells. Simultaneously, the release of CDs initiates fluorescence, which has been previously suppressed by the molecular structure. 5 FU exhibits substantial anticancer activity, as evidenced by its favourable release profile under acidic conditions (60.42 %). Cellular uptake and viability assessments of HeLa (cervical cancer) and MCF-7 (breast cancer) cell lines, as well as non-cancerous L929 cells, have produced promising results, particularly for folate rich HeLa cells. This study illustrates a novel method of bio-sensing by fluorescent CDs and targeted drug delivery, which is combined with MOF mediated destruction of cancer cells and Fenton reactions.

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用于检测和破坏癌细胞的碳点诱导铁 MOF 的制备和特性分析

开发一种既能靶向癌细胞又能最大限度减少化疗副作用的有效给药系统仍然是癌症治疗领域的一项重大挑战。这项工作介绍了一种生物可降解金属有机框架（MOF）支架系统，该系统通过碳点（CD）增强，可促进药物的精确输送并追踪药物在癌细胞内的路径。我们合成并还原了一种铁基 MOF（rMOF），使其能在癌细胞中进行芬顿反应。我们使用 1-乙基-3-（3-二甲氨基丙基）碳二亚胺和 N-羟基琥珀酰亚胺（EDC-NHS）交联化学方法将叶酸衍生的碳点与 rMOF 交联，以提供生物传感能力，并随后将抗癌药物 5 氟尿嘧啶（5 FU）纳入该系统，以获得靶向抗癌活性。由于胺基质子化作用，rMOF-CD 连接在酸性环境中会被破坏，从而导致 MOF 结构解体并释放出 Fe2+ 离子。这些离子能与细胞中的 H2O2 发生 Fenton 反应，从而摧毁癌细胞。与此同时，CD 的释放也引发了荧光，而这种荧光之前一直被分子结构所抑制。5 FU 具有很强的抗癌活性，其在酸性条件下的良好释放曲线（60.42%）就证明了这一点。对 HeLa（宫颈癌）和 MCF-7（乳腺癌）细胞系以及非癌细胞 L929 进行的细胞吸收和存活率评估结果令人鼓舞，尤其是富含叶酸的 HeLa 细胞。这项研究展示了一种通过荧光 CD 进行生物传感和靶向给药的新方法，该方法与 MOF 介导的癌细胞破坏和 Fenton 反应相结合。

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来源期刊

Nano-Structures & Nano-Objects Physics and Astronomy-Condensed Matter Physics

CiteScore

9.20

自引率

0.00%

发文量

审稿时长

22 days

期刊介绍： Nano-Structures & Nano-Objects is a new journal devoted to all aspects of the synthesis and the properties of this new flourishing domain. The journal is devoted to novel architectures at the nano-level with an emphasis on new synthesis and characterization methods. The journal is focused on the objects rather than on their applications. However, the research for new applications of original nano-structures & nano-objects in various fields such as nano-electronics, energy conversion, catalysis, drug delivery and nano-medicine is also welcome. The scope of Nano-Structures & Nano-Objects involves: -Metal and alloy nanoparticles with complex nanostructures such as shape control, core-shell and dumbells -Oxide nanoparticles and nanostructures, with complex oxide/metal, oxide/surface and oxide /organic interfaces -Inorganic semi-conducting nanoparticles (quantum dots) with an emphasis on new phases, structures, shapes and complexity -Nanostructures involving molecular inorganic species such as nanoparticles of coordination compounds, molecular magnets, spin transition nanoparticles etc. or organic nano-objects, in particular for molecular electronics -Nanostructured materials such as nano-MOFs and nano-zeolites -Hetero-junctions between molecules and nano-objects, between different nano-objects & nanostructures or between nano-objects & nanostructures and surfaces -Methods of characterization specific of the nano size or adapted for the nano size such as X-ray and neutron scattering, light scattering, NMR, Raman, Plasmonics, near field microscopies, various TEM and SEM techniques, magnetic studies, etc .