ProT-Patch: A Smart Coated Polymeric Microneedle Enables Noninvasive Protein Delivery and Reprogramming of Epidermal Skin Identity

IF 11.3 1区化学 Q1 CHEMISTRY, PHYSICAL

ACS Catalysis Pub Date : 2024-10-07 DOI:10.1021/acsmaterialslett.4c0160910.1021/acsmaterialslett.4c01609

Attiya A. Abbas, Wildan Hanif, Isobel Steer, Erol Hasan, Oliver Teenan, Mo Akhavani, Khaled Mutabagani, Benjamin D. Almquist, Claire A. Higgins and Dana Alsulaiman*,

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引用次数: 0

Abstract

Microneedles have emerged as transformative devices for noninvasive drug delivery through skin; however, reported platforms suffer poor skin penetration, dosage inaccuracy, and/or complex fabrication. Herein, we develop and validate a smart pH-responsive polymeric microneedle (proT-patch) for efficient drug delivery to the basal epidermal layer of the skin. The microneedle base offers high stiffness, while a variable needle height design eludes the “bed of nails” effect. With its stimuli-responsiveness, the microneedle coating enables drug release under specific pH conditions, enhancing dosage accuracy and minimizing biowaste. The performance of proT-patch is validated using ex vivo human skin by delivering Wnt5a, which switches on Keratin-9 expression, and is not expressed in nonplantar skin. Administering Wnt5a-loaded proT-patch to skin enhanced Keratin-9 expression in basal epidermal keratinocytes compared to topical and unloaded controls. With its facile fabrication, versatility, and exceptional performance, proT-patch shows immense potential as a next-generation, noninvasive tool for therapeutics, cosmetics, and vaccine delivery.

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ProT-Patch：智能涂层聚合物微针可实现无创蛋白质传输和表皮皮肤身份重编程

微针已成为通过皮肤进行非侵入性给药的变革性设备；然而，已报道的平台存在皮肤穿透性差、剂量不准确和/或制造复杂等问题。在本文中，我们开发并验证了一种智能 pH 值响应型聚合物微针（proT-patch），用于向皮肤表皮基底层高效给药。微针基底具有高硬度，而可变针高设计则避免了 "钉床 "效应。微针涂层具有刺激响应性，可在特定的 pH 值条件下释放药物，从而提高剂量的准确性并最大限度地减少生物垃圾。proT-patch 的性能通过体内外人体皮肤进行了验证，它能传递 Wnt5a，而 Wnt5a 能开启角蛋白-9 的表达，但在非跖皮肤中并不表达。与局部使用和未使用的对照组相比，在皮肤上涂抹负载 Wnt5a 的 proT-patch 可增强基底表皮角质细胞中 Keratin-9 的表达。ProT-patch 制作简便、用途广泛、性能卓越，显示出作为下一代非侵入性治疗、美容和疫苗递送工具的巨大潜力。

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来源期刊

ACS Catalysis CHEMISTRY, PHYSICAL-

CiteScore

20.80

自引率

6.20%

发文量

1253

审稿时长

1.5 months

期刊介绍： ACS Catalysis is an esteemed journal that publishes original research in the fields of heterogeneous catalysis, molecular catalysis, and biocatalysis. It offers broad coverage across diverse areas such as life sciences, organometallics and synthesis, photochemistry and electrochemistry, drug discovery and synthesis, materials science, environmental protection, polymer discovery and synthesis, and energy and fuels. The scope of the journal is to showcase innovative work in various aspects of catalysis. This includes new reactions and novel synthetic approaches utilizing known catalysts, the discovery or modification of new catalysts, elucidation of catalytic mechanisms through cutting-edge investigations, practical enhancements of existing processes, as well as conceptual advances in the field. Contributions to ACS Catalysis can encompass both experimental and theoretical research focused on catalytic molecules, macromolecules, and materials that exhibit catalytic turnover.