Enhanced VCA Storage: A Pilot Study Demonstrating Supercooling in Orthotopic Rodent Hindlimb Transplantation

IF 0.8 4区医学 Q4 IMMUNOLOGY

Transplantation proceedings Pub Date : 2024-11-01 DOI:10.1016/j.transproceed.2024.10.006

Irina Filz von Reiterdank , McLean S. Taggart , Michelle E. McCarthy , Antonia T. Dinicu , Basak E. Uygun , J. Henk Coert , Aebele B. Mink van der Molen , Korkut Uygun

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引用次数: 0

Abstract

The field of vascularized composite allograft (VCA) transplantation has seen steady, rapid growth, with new innovations driving the evolution from experimental procedures to more standardized therapies. With this expansion comes challenges with graft allocation, preservation, and postoperative graft rejection. Here, we outline the first example of subzero nonfreezing (SZNF), supercooled storage of a whole rat hindlimb with orthotopic transplantation. Rat hindlimbs were procured, loaded, and supercooled for 48 hours at –4°C (n = 4), after which, they were recovered. The loading and recovery phase were performed using subnormothermic machine perfusion (SNMP) during which viability markers (glucose and oxygen consumption, lactate, and resistance) were tracked. Control limbs underwent static cold storage (SCS). After ex vivo validation, the model was piloted in a transplant model, comparing 48 hours of SZNF (n = 1), 48 hours of SCS (n = 1), and 72 hours of SCS (n = 1), which demonstrated no survival beyond postoperative day 4 in the SCS models, and survival until the end of study (postoperative day [POD] 28) in the SZNF model. This study demonstrates the promise of this model in future studies on long-term VCA preservation.

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增强 VCA 储存：在同种异位啮齿动物后肢移植中展示过冷的试点研究。

血管化复合异体移植物（VCA）移植领域一直保持着稳定、快速的增长，新的创新技术推动着实验性手术向更标准化的疗法发展。随着这一领域的扩展，移植物的分配、保存和术后移植物排斥反应等问题也随之而来。在这里，我们概述了第一个零度以下非冷冻（SZNF）、超冷储存整条大鼠后肢并进行正位移植的实例。我们采集、装载大鼠后肢，并在零下 4 摄氏度的条件下超低温保存 48 小时（n = 4），然后将其复原。装载和恢复阶段使用亚恒温机灌注（SNMP），在此期间跟踪存活标志物（葡萄糖和氧气消耗、乳酸和阻力）。对照组肢体进行静态冷藏（SCS）。经过体内外验证后，该模型在移植模型中进行了试验，比较了 48 小时 SZNF（n = 1）、48 小时 SCS（n = 1）和 72 小时 SCS（n = 1），结果表明 SCS 模型在术后第 4 天后无存活，而 SZNF 模型则存活至研究结束（术后第 [POD] 28 天）。这项研究表明，该模型有望在未来的长期 VCA 保存研究中发挥作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Transplantation proceedings 医学-免疫学

CiteScore

1.70

自引率

0.00%

发文量

502

审稿时长

60 days

期刊介绍： Transplantation Proceedings publishes several different categories of manuscripts, all of which undergo extensive peer review by recognized authorities in the field prior to their acceptance for publication. The first type of manuscripts consists of sets of papers providing an in-depth expression of the current state of the art in various rapidly developing components of world transplantation biology and medicine. These manuscripts emanate from congresses of the affiliated transplantation societies, from Symposia sponsored by the Societies, as well as special Conferences and Workshops covering related topics. Transplantation Proceedings also publishes several special sections including publication of Clinical Transplantation Proceedings, being rapid original contributions of preclinical and clinical experiences. These manuscripts undergo review by members of the Editorial Board. Original basic or clinical science articles, clinical trials and case studies can be submitted to the journal?s open access companion title Transplantation Reports.