Immunomodulatory Potential of Melatonin in Immunosuppression: An in-vivo Study.

Aamina Quddus Qureshi, Saghir Ahmed Jafri, Ambreen Tauseef, Waqar Ahmed Siddiqui, Amna Irum, Ayela Eman Zia
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Abstract

Objective: To assess the immunomodulatory effects of melatonin on the acquired immunity of immunosuppressed male Wistar rats by checking Interleukin 6 (IL-6) levels, total leucocyte counts (TLC), and differential leucocyte counts.

Study design: Experimental study. Place and Duration of the Study: Animal laboratory, CMH Lahore Medical College, from June to October 2023.

Methodology: Fifty male Wistar rats, weighing 180g to 200g, were included in this study with 10 rats in each of the five groups namely cyclophosphamide (CP), CP + melatonin, CP + immunomodulator, melatonin-only, and control. A calculated dose of CP was administered intraperitoneally for 30 days (from 13/06/23 to 13/07/23) to each group except the control groups. After this, the experimental group was given melatonin CP + Melatonin for 7 days (from 14/07/23 to 20/07/23). CP + Immunomodulatory group was kept for comparison of immunomodulatory effects. Blood samples were drawn from all 5 groups. IL-6 was estimated through ELISA. Other parameters assessed were TLC and absolute differential leucocyte counts.

Results: Melatonin increased IL-6 levels significantly (p = 0.042) as well as the TLC levels (p <0.001) compared to the immuno-suppressed CP group. Melatonin was seen to have an upregulation of IL-6 levels in immunosuppression compared to the administration of immunomodulator preparation (p = 0.506) which was not as effective. Administration of melatonin significantly increased the TLC, neutrophil, lymphocyte, monocyte, and eosinophil count compared to known immunomodulators.

Conclusion: Melatonin as a supplement may have some role in activating multiple immune response processes in immune-depressed states. It was also established that it allows quick recovery of cell components from immunosuppressed states.

Key words: Melatonin, Immunomodulation, Rats, Interleukin-6, Acquired immunity, Cyclophosphamide.

褪黑素在免疫抑制中的免疫调节潜力:体内研究
目的通过检测白细胞介素6(IL-6)水平、白细胞总数(TLC)和差异白细胞计数,评估褪黑素对免疫抑制雄性Wistar大鼠获得性免疫的免疫调节作用:实验研究。研究地点和时间:2023 年 6 月至 10 月,拉合尔医学院动物实验室:研究对象:50 只雄性 Wistar 大鼠,体重 180 克至 200 克,分为环磷酰胺 (CP)、CP + 褪黑激素、CP + 免疫调节剂、纯褪黑激素和对照组五组,每组 10 只。除对照组外,每组均腹腔注射计算剂量的 CP 30 天(13/06/23 至 13/07/23)。之后,实验组给予褪黑素 CP + 褪黑素 7 天(从 14/07/23 到 20/07/23)。保留 CP + 免疫调节组,以比较免疫调节效果。从所有 5 组中抽取血液样本。通过 ELISA 测定 IL-6。其他评估参数包括TLC和绝对差异白细胞计数:结果:褪黑素能显著提高 IL-6 水平(p = 0.042)和 TLC 水平(p 结论:褪黑素作为一种补充剂,可能对人体有一定的调节作用:褪黑素作为一种补充剂,可能在激活免疫抑制状态下的多种免疫反应过程中发挥一定作用。此外,褪黑素还能使免疫抑制状态下的细胞成分快速恢复:褪黑素 免疫调节 大鼠 白细胞介素-6 后天免疫 环磷酰胺
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