Antibiotic biotransformation potential of biofilms in streams receiving treated wastewater effluent: Biodegradation mechanism and bacterial community structure

Abstract

Antibiotics are a widely distributed and effective antibacterial agents. Human medical treatment and livestock aquaculture are major sources of antibiotics in aquatic ecosystems, potentially damaging the biofilms that are the foundation of stream food webs. In this study, we conducted antibiotic biotransformation experiments using biofilms cultured in streams upstream and downstream of a wastewater treatment plant outlet to distinguish different fate processes of antibiotics in biofilms. It was found that stream biofilms have biotransformation potential mainly for specific sulfonamide antibiotics. Flavobacterium and Dyadobacter were identified to be associated with biofilm biotransformation of antibiotics by 16S rDNA sequencing. Besides, microorganisms released from treated wastewater integrated into downstream biofilm communities, thereby enhancing the biotransformation potential of downstream biofilm communities compared to upstream biofilm. These findings enriched the understanding of the biotransformation of micropollutants by stream biofilms, and to thus promote the development of biofilm-based monitoring technologies.