Antioxidant properties, anti-nutritive and toxic factors of Terminalia sericea in Onderstepoort.

IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES
Tirelo Matlala, Rejoice B Maseko, Kedibone G Kgosana
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Abstract

Anti-nutritive and toxic factors in plants adversely affect animal health and production. Hence, the aims of the study were to (1) carry out qualitative and quantitative analysis of these factors in Terminalia sericea that grows abundantly in Onderstepoort; (2) evaluate an affordable, efficient and reliable method that reduces these factors and (3) evaluate antioxidant properties of the phenolic compounds. Thus, sequential organic and aqueous extractions were carried out on T. sericea leaves and feed pellets. Extracts were subjected to qualitative analysis to confirm the presence of 11 phytochemicals. While quantitative analysis of nine phytochemicals was carried out on spectrophotometry, the phenolic extracts were further tested for antioxidant activity on 2,2-diphenyl-picrylhydrazyl (DPPH) assay where gallic acid (GA), quercetin (Q) and ascorbic acid (AA) were used as standards. The contents of GA, Q and AA in the phenolic extracts were determined on high-performance liquid chromatography (HPLC). Aqueous extraction was an effective method as most phytochemicals were extracted. Nine phytochemicals were qualitatively analysed in T. sericea. The highest contents of 1.97% and 2.37 ± 0.077 mg/g gallic acid equivalent with a significant difference (p  0.05) were determined in oxalates and phenolics, respectively. Furthermore, 83.1% DPPH inhibition (IC50 = 0.0432 µg/mL) was determined relative to the standards (85.27% - 85.96%; IC50 = 0.000009 µg/mL - 0.00042 µg/mL). Subsequently, HPLC results showed that T. sericea contained high concentration of AA (62.5 ± 2.13 µg/mL) to confirm antioxidant properties of the leaves.Contribution: Though T. sericea leaves have antioxidant activities, the alkaloid contents may pose a threat to the livestock. Therefore, farmers around Onderstepoort should protect their livestock from feeding on these leaves.

Onderstepoort 的蚕豆抗氧化性、抗营养性和毒性因子。
植物中的抗营养因子和有毒因子会对动物健康和生产造成不利影响。因此,这项研究的目的是:(1)对生长在 Onderstepoort 的绢毛茶中的这些因子进行定性和定量分析;(2)评估一种经济、高效、可靠的方法,以减少这些因子;(3)评估酚类化合物的抗氧化特性。因此,对 T. sericea 的叶子和饲料颗粒进行了有机和水提取。对萃取物进行了定性分析,以确认 11 种植物化学物质的存在。在对 9 种植物化学物质进行分光光度法定量分析的同时,以没食子酸(GA)、槲皮素(Q)和抗坏血酸(AA)为标准,用 2,2-二苯基-苦基肼(DPPH)法进一步检测酚类提取物的抗氧化活性。高效液相色谱法(HPLC)测定了酚类提取物中的没食子酸(GA)、槲皮素(Q)和抗坏血酸(AA)含量。水提取是一种有效的方法,因为大多数植物化学物质都被提取出来了。对 T. sericea 中的 9 种植物化学物质进行了定性分析。草酸盐和酚类物质的含量最高,分别为 1.97% 和 2.37 ± 0.077 mg/g 没食子酸当量,差异显著(P 0.05)。此外,与标准(85.27% - 85.96%;IC50 = 0.000009 µg/mL - 0.00042 µg/mL)相比,DPPH 抑制率为 83.1%(IC50 = 0.0432 µg/mL)。随后,高效液相色谱结果表明,T. sericea 含有高浓度 AA(62.5 ± 2.13 µg/mL),证实了叶子的抗氧化特性:虽然蚕沙叶具有抗氧化活性,但其生物碱含量可能会对牲畜造成威胁。因此,Onderstepoort 附近的农民应该保护他们的牲畜,不要让牲畜采食这些叶子。
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来源期刊
CiteScore
4.30
自引率
0.00%
发文量
13
审稿时长
16 weeks
期刊介绍: The Onderstepoort Journal of Veterinary Research, is the official publication of the Onderstepoort Veterinary Institute. While it considers submissions from any geographic region, its focus is on Africa and the infectious and parasitic diseases and disease vectors that affect livestock and wildlife on the continent.
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