Virulence and Antimicrobial Resistance Patterns of Salmonella spp. Recovered From Migratory and Captive Wild Birds.

IF 1.8 3区农林科学 Q2 VETERINARY SCIENCES

Veterinary Medicine and Science Pub Date : 2024-11-01 DOI:10.1002/vms3.70102

Ruhena Begum, Nilima Akther Asha, Diponkar Chandra Chanda Dipu, Milton Roy, Asikur Rahman, Md Shahidur Rahman Chowdhury, Hemayet Hossain, Md Rafiqul Islam, Md Bashir Uddin, Md Mahfujur Rahman, Md Mukter Hossain

{"title":"Virulence and Antimicrobial Resistance Patterns of Salmonella spp. Recovered From Migratory and Captive Wild Birds.","authors":"Ruhena Begum, Nilima Akther Asha, Diponkar Chandra Chanda Dipu, Milton Roy, Asikur Rahman, Md Shahidur Rahman Chowdhury, Hemayet Hossain, Md Rafiqul Islam, Md Bashir Uddin, Md Mahfujur Rahman, Md Mukter Hossain","doi":"10.1002/vms3.70102","DOIUrl":null,"url":null,"abstract":"Background: Salmonella spp., especially those are resistant to extended-spectrum β-lactamase (ESBL), are considered as major concern to global health due to their emergence and dissemination.Aim: The aim of this study was to investigate the virulence and antimicrobial resistance (AMR) profile of Salmonella spp. from migratory and captive wild birds.Method: A total 262 faecal samples were collected, and the identification of Salmonella spp. was carried out using a standard culture and PCR as well as molecular detection of virulence and AMR genes.Results: The overall prevalence of Salmonella was determined to be 30.92% (95% CI = 25.63-36.75). Migratory birds exhibited highest prevalence (38.10%), whereas wild birds in captivity showed a lower prevalence (23.40%). The agfA gene was detected at a higher rate at 24.69%. Salmonella spp. exhibited 100% resistance to tetracycline, followed by 58% ampicillin and 46% streptomycin. In addition, there was a resistance rate to ceftriaxone of 17% and to colistin sulphate of 25%. Interestingly, levofloxacin alone displayed 100% sensitivity across all isolates, while ciprofloxacin and azithromycin showed 73% and 64% sensitivity, respectively. The MAR index was 0.25 and 0.42, and 74.07% of all isolates showed multidrug resistance (MDR). It was shown that migratory and captive wild birds contained ESBL genes blaTEM (94.34% and 49.06%) and blaSHV (13.33% and 10%), respectively. Genes responsible for sulphonamide (sul1) resistance were detected in 13.33% and 79% of wild and migratory birds, respectively.Conclusion: Salmonella has been found in captive wild and migratory birds and could act as reservoirs for the transmission of MDR and ESBL bacteria.","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11533216/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Medicine and Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1002/vms3.70102","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Salmonella spp., especially those are resistant to extended-spectrum β-lactamase (ESBL), are considered as major concern to global health due to their emergence and dissemination.

Aim: The aim of this study was to investigate the virulence and antimicrobial resistance (AMR) profile of Salmonella spp. from migratory and captive wild birds.

Method: A total 262 faecal samples were collected, and the identification of Salmonella spp. was carried out using a standard culture and PCR as well as molecular detection of virulence and AMR genes.

Results: The overall prevalence of Salmonella was determined to be 30.92% (95% CI = 25.63-36.75). Migratory birds exhibited highest prevalence (38.10%), whereas wild birds in captivity showed a lower prevalence (23.40%). The agfA gene was detected at a higher rate at 24.69%. Salmonella spp. exhibited 100% resistance to tetracycline, followed by 58% ampicillin and 46% streptomycin. In addition, there was a resistance rate to ceftriaxone of 17% and to colistin sulphate of 25%. Interestingly, levofloxacin alone displayed 100% sensitivity across all isolates, while ciprofloxacin and azithromycin showed 73% and 64% sensitivity, respectively. The MAR index was 0.25 and 0.42, and 74.07% of all isolates showed multidrug resistance (MDR). It was shown that migratory and captive wild birds contained ESBL genes blaTEM (94.34% and 49.06%) and blaSHV (13.33% and 10%), respectively. Genes responsible for sulphonamide (sul1) resistance were detected in 13.33% and 79% of wild and migratory birds, respectively.

Conclusion: Salmonella has been found in captive wild and migratory birds and could act as reservoirs for the transmission of MDR and ESBL bacteria.

查看原文本刊更多论文

从迁徙和圈养野生鸟类中发现的沙门氏菌的毒性和抗菌药耐药性模式。

背景：沙门氏菌属，尤其是对广谱β-内酰胺酶（ESBL）具有耐药性的沙门氏菌属，因其出现和传播而被认为是全球健康的主要问题：方法：共收集了 262 份粪便样本，采用标准培养和 PCR 方法对沙门氏菌进行鉴定，并对毒力基因和 AMR 基因进行分子检测：结果：沙门氏菌的总体流行率为 30.92%（95% CI = 25.63-36.75）。候鸟的发病率最高（38.10%），而人工饲养的野鸟发病率较低（23.40%）。agfA 基因的检出率较高，为 24.69%。沙门氏菌属对四环素的抗药性为 100%，其次是 58%的氨苄西林和 46%的链霉素。此外，对头孢曲松的耐药率为 17%，对硫酸可乐定的耐药率为 25%。有趣的是，单用左氧氟沙星对所有分离菌株的敏感性为 100%，而环丙沙星和阿奇霉素的敏感性分别为 73% 和 64%。MAR指数分别为0.25和0.42，74.07%的分离株表现出多药耐药性（MDR）。研究表明，候鸟和人工饲养的野生鸟类分别含有 94.34% 和 49.06% 的 ESBL 基因 blaTEM 和 13.33% 和 10%的 ESBL 基因 blaSHV。在13.33%和79%的野生鸟类和候鸟中分别检测到对磺胺（sul1）产生抗性的基因：结论：在人工饲养的野生鸟类和候鸟中发现了沙门氏菌，它们可能成为 MDR 和 ESBL 细菌的传播源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Veterinary Medicine and Science Veterinary-General Veterinary

CiteScore

3.00

自引率

0.00%

发文量

296

期刊介绍： Veterinary Medicine and Science is the peer-reviewed journal for rapid dissemination of research in all areas of veterinary medicine and science. The journal aims to serve the research community by providing a vehicle for authors wishing to publish interesting and high quality work in both fundamental and clinical veterinary medicine and science. Veterinary Medicine and Science publishes original research articles, systematic reviews, meta-analyses, and research methods papers, along with invited editorials and commentaries. Original research papers must report well-conducted research with conclusions supported by the data presented in the paper. We aim to be a truly global forum for high-quality research in veterinary medicine and science, and believe that the best research should be published and made widely accessible as quickly as possible. Veterinary Medicine and Science publishes papers submitted directly to the journal and those referred from a select group of prestigious journals published by Wiley-Blackwell. Veterinary Medicine and Science is a Wiley Open Access journal, one of a new series of peer-reviewed titles publishing quality research with speed and efficiency. For further information visit the Wiley Open Access website.