Development and Application of an Indirect Enzyme-Linked Immunosorbent Assay Based on a Recombinant Matrix Protein for the Serological Study of Porcine Deltacoronavirus in Mexican Pigs.

IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES
Francisco Jesus Castañeda Montes, José Luis Cerriteño Sánchez, Julieta Sandra Cuevas-Romero, María Azucena Castañeda Montes, Susana Mendoza Elvira
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引用次数: 0

Abstract

Porcine deltacoronavirus (PDCoV) is an infectious disease that causes diarrhoea in pigs of different ages; however, piglets are more susceptible. PDCoV was first reported in 2012 in China and Hong Kong. Later, it was first reported in the USA in 2014 and in Mexico in 2019. Several studies have shown that M protein is highly conserved and, therefore, suitable for diagnostic systems. In this study, for the first time, an indirect enzyme-linked immunosorbent assay (iELISA) based on a recombinant M protein (rM-PDCoV) was developed to evaluate the seroprevalence of PDCoV in four states in Mexico. High sensitivity (83%) and specificity (100%) were observed for the iELISA. The kappa index calculated a nearly perfect agreement (0.8831) compared to the Western blot (gold standard test), suggesting acceptable statistical value support. In this study, 50.38% of the serum samples from backyard pigs were PDCoV-positive. The serological comparison showed that PDCoV/PEDV coinfections occurred in 31.98% of the analysed sera. These results can enrich our understanding of how this virus spreads and enable the evaluation of PDCoV infections. Moreover, it highlights the importance of continually investigating the seroprevalence of PDCoV in Mexico because there is also no information about the current prevalence of the disease.

基于重组基质蛋白的间接酶联免疫吸附测定法在墨西哥猪猪瘟病毒血清学研究中的开发与应用
猪瘟病毒(PDCoV)是一种传染病,可导致不同年龄的猪腹泻,但仔猪更易感染。PDCoV 于 2012 年首次在中国和香港报告。随后,2014 年和 2019 年分别在美国和墨西哥首次报道。多项研究表明,M 蛋白具有高度保守性,因此适用于诊断系统。本研究首次开发了一种基于重组 M 蛋白(rM-PDCoV)的间接酶联免疫吸附试验(iELISA),用于评估墨西哥四个州的 PDCoV 血清流行率。iELISA 的灵敏度(83%)和特异性(100%)都很高。卡帕指数(kappa index)与西方印迹(金标检测)相比,计算出的结果几乎完全一致(0.8831),这表明统计值支持是可以接受的。在这项研究中,50.38% 的散养猪血清样本呈 PDCoV 阳性。血清学比较显示,31.98% 的分析血清出现 PDCoV/PEDV 共感染。这些结果丰富了我们对该病毒传播途径的了解,并有助于对 PDCoV 感染进行评估。此外,它还强调了继续调查墨西哥 PDCoV 血清流行率的重要性,因为目前还没有关于该疾病流行率的信息。
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来源期刊
Veterinary Medicine and Science
Veterinary Medicine and Science Veterinary-General Veterinary
CiteScore
3.00
自引率
0.00%
发文量
296
期刊介绍: Veterinary Medicine and Science is the peer-reviewed journal for rapid dissemination of research in all areas of veterinary medicine and science. The journal aims to serve the research community by providing a vehicle for authors wishing to publish interesting and high quality work in both fundamental and clinical veterinary medicine and science. Veterinary Medicine and Science publishes original research articles, systematic reviews, meta-analyses, and research methods papers, along with invited editorials and commentaries. Original research papers must report well-conducted research with conclusions supported by the data presented in the paper. We aim to be a truly global forum for high-quality research in veterinary medicine and science, and believe that the best research should be published and made widely accessible as quickly as possible. Veterinary Medicine and Science publishes papers submitted directly to the journal and those referred from a select group of prestigious journals published by Wiley-Blackwell. Veterinary Medicine and Science is a Wiley Open Access journal, one of a new series of peer-reviewed titles publishing quality research with speed and efficiency. For further information visit the Wiley Open Access website.
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