Pgmiox mediates stress response and plays a critical role for pathogenicity in Pyrenophora graminea, the agent of barley leaf stripe

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2024-10-28 DOI:10.1016/j.plantsci.2024.112308

Ming Guo , Erjing Si , Jingjing Hou , Lirong Yao , Juncheng Wang , Yaxiong Meng , Xiaole Ma , Baochun Li , Huajun Wang

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引用次数: 0

Abstract

Barley leaf stripe is an important disease caused by Pyenophora graminea that affects barley yields in the world. Ascorbic acid (AsA) interacts with key elements of a complex network orchestrating plant defense mechanisms, thereby influencing the outcome of plant-pathogen interaction. Myo-inositol oxygenase (MIOX) is a pivotal enzyme involved in plants development and environmental stimuli. However, MIOX has described functions in plants but has not been characterized in fungi. In this study, we characterized the Pgmiox gene in P. graminea pathogenesis through annotated on the metabolic pathway of ascorbic acid aldehyde. Our analysis suggested that the Pgmiox protein had a typical conserved MIOX domain. Multiple alignment analysis indicated that the P. graminea MIOX orthologue clustered with MIOX proteins of Pyrenophora species. RNA interference successfully reduced transcript abundance of Pgmiox in six transformant lines compared to wild type, and the transformants were further less virulent on the host plant barley. Transformants of Pgmiox had significant reductions in vegetative growth and pathogenicity, which had increased resistance to tebuconazole and carbendazim. In addition, Pgmiox is associated with ionic, drought, osmotic, oxidative, and heavy metal stress tolerance in P. graminea. In conclusion, our findings reveal that Pgmiox may be widely utilized by fungi to enhance pathogenesis and holds significant potential for the development of durable P. graminea resistance through genetic modifications.

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Pgmiox 能介导应激反应，并对大麦叶斑病病原体禾谷轮枝霉的致病性起关键作用。

大麦叶斑病是由禾本科扁孢菌（Pyenophora graminea）引起的一种影响全球大麦产量的重要病害。抗坏血酸（AsA）与协调植物防御机制的复杂网络中的关键元素相互作用，从而影响植物与病原体相互作用的结果。肌醇加氧酶（MIOX）是参与植物发育和环境刺激的关键酶。然而，MIOX 在植物中的功能已被描述，但在真菌中的功能还没有表征。在本研究中，我们通过对抗坏血酸醛代谢途径的注释，确定了 Pgmiox 基因在革兰氏菌致病过程中的特性。我们的分析表明，Pgmiox 蛋白具有典型的保守 MIOX 结构域。多重比对分析表明，革兰氏菌的 MIOX 直向同源物与拟杆菌的 MIOX 蛋白聚集在一起。与野生型相比，RNA 干扰成功地降低了六个转化株系中 Pgmiox 的转录本丰度，转化株系对宿主植物大麦的毒性进一步降低。Pgmiox 转化株的无性生长和致病性显著降低，对戊唑醇和多菌灵的抗性增强。此外，Pgmiox 还与禾谷类真菌的离子、干旱、渗透、氧化和重金属胁迫耐受性有关。总之，我们的研究结果表明，真菌可能广泛利用 Pgmiox 来增强致病机理，并具有通过基因修饰开发禾谷类真菌持久抗性的巨大潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.