Autoinducer-2 relieves soil stress-induced dormancy of Bacillus velezensis by modulating sporulation signaling.

IF 7.8 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

npj Biofilms and Microbiomes Pub Date : 2024-11-03 DOI:10.1038/s41522-024-00594-6

Qin Xiong, Huihui Zhang, Xia Shu, Xiting Sun, Haichao Feng, Zhihui Xu, Ákos T Kovács, Ruifu Zhang, Yunpeng Liu

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引用次数: 0

Abstract

The collective behavior of bacteria is regulated by quorum sensing (QS). Autoinducer-2 (AI-2) is a common QS signal that regulates the behavior of both Gram-positive and Gram-negative bacteria. Despite the plethora of processes described to be influenced by AI-2 in diverse Gram-negative bacteria, the AI-2-regulated processes in Bacilli are relatively unexplored. Here, we describe a novel function for AI-2 in Bacillus velezensis SQR9 related to the sporulation. AI-2 inhibited the initiation of sporulation through the phosphatase RapC and the DNA binding regulator ComA. Using biochemistry experiments, we demonstrated that AI-2 interacts with RapC to stimulate its binding to ComA, which leads to an inactive ComA and subsequently a sporulation inhibition. The AI-2 molecule could be shared across species for inhibiting Bacillus sporulation and it also plays the same role in different soil conditions. Our study revealed a novel function and regulatory mechanism of AI-2 in inhibiting sporulation in Bacilli.

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自诱导因子-2通过调节孢子信号转导缓解土壤胁迫诱导的韦氏芽孢杆菌休眠。

细菌的集体行为受法定量感应（QS）调控。自诱导子-2（AI-2）是一种常见的 QS 信号，可调节革兰氏阳性和革兰氏阴性细菌的行为。尽管在各种革兰氏阴性细菌中，AI-2 影响了大量的过程，但在芽孢杆菌中，AI-2 调控的过程却相对较少。在这里，我们描述了 AI-2 在韦氏芽孢杆菌 SQR9 中与孢子形成有关的一种新功能。AI-2 通过磷酸酶 RapC 和 DNA 结合调节因子 ComA 抑制孢子的形成。我们利用生化实验证明，AI-2 与 RapC 相互作用，刺激 RapC 与 ComA 结合，导致 ComA 失去活性，进而抑制孢子的产生。AI-2 分子在抑制芽孢杆菌孢子发生方面可以跨物种共享，而且在不同的土壤条件下也发挥着相同的作用。我们的研究揭示了 AI-2 在抑制芽孢杆菌孢子形成中的新功能和调控机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

npj Biofilms and Microbiomes Immunology and Microbiology-Microbiology

CiteScore

12.10

自引率

3.30%

发文量

审稿时长

9 weeks

期刊介绍： npj Biofilms and Microbiomes is a comprehensive platform that promotes research on biofilms and microbiomes across various scientific disciplines. The journal facilitates cross-disciplinary discussions to enhance our understanding of the biology, ecology, and communal functions of biofilms, populations, and communities. It also focuses on applications in the medical, environmental, and engineering domains. The scope of the journal encompasses all aspects of the field, ranging from cell-cell communication and single cell interactions to the microbiomes of humans, animals, plants, and natural and built environments. The journal also welcomes research on the virome, phageome, mycome, and fungome. It publishes both applied science and theoretical work. As an open access and interdisciplinary journal, its primary goal is to publish significant scientific advancements in microbial biofilms and microbiomes. The journal enables discussions that span multiple disciplines and contributes to our understanding of the social behavior of microbial biofilm populations and communities, and their impact on life, human health, and the environment.