Exploring the Interplay of Wavelength, Quantum Yield, and Penetration Depth in In Vivo Fluorescence Imaging.

IF 2.6 4区 化学 Q2 BIOCHEMICAL RESEARCH METHODS
Meital Harel, Rinat Ankri
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引用次数: 0

Abstract

The intricate interplay between the irradiation wavelength, the fluorophore quantum yield (QY) and penetration depth profoundly influences the efficacy of in vivo fluorescence imaging in various applications. Understanding the complex behavior of fluorescence in vivo, specifically how variations in wavelength affect the QY of commonly used dyes and the depth of imaging is crucial for optimizing fluorescence imaging techniques, as it directly impacts the accuracy and efficiency of imaging in biological tissues. In our study, we explore these dynamics through Monte Carlo simulations conducted under conditions reflective of wide-field fluorescence imaging, examining how variations in wavelength impact the dye's QY and depth of imaging, and consequently, the fluorescence behavior. A transition in the exponential decay of the emission depth exponent is observed around the 500-600 nm range, indicating varying degrees of influence of depth on the fluorescence emission. The analysis of the fluorophore's QY reveals wavelength-dependent variations, with the most significant impact observed in the 600-700 nm range. Moreover, we continued our investigation to explore multiplexing, unveiling insights into the spacing between identical spots in multiplexing images across various depths and wavelengths.

探索体内荧光成像中波长、量子产率和穿透深度的相互作用
照射波长、荧光团量子产率(QY)和穿透深度之间错综复杂的相互作用深刻影响着各种应用中体内荧光成像的效果。了解体内荧光的复杂行为,特别是波长变化如何影响常用染料的量子产率和成像深度,对于优化荧光成像技术至关重要,因为它直接影响生物组织成像的准确性和效率。在我们的研究中,我们通过在反映宽场荧光成像的条件下进行蒙特卡洛模拟来探索这些动态变化,研究波长变化如何影响染料的 QY 和成像深度,进而影响荧光行为。在 500-600 纳米范围内观察到发射深度指数衰减的转变,表明深度对荧光发射的影响程度不同。对荧光团 QY 的分析表明,其变化与波长有关,在 600-700 纳米范围内观察到的影响最为显著。此外,我们还继续研究了多路复用,揭示了不同深度和波长的多路复用图像中相同点之间的间距。
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来源期刊
Journal of Fluorescence
Journal of Fluorescence 化学-分析化学
CiteScore
4.60
自引率
7.40%
发文量
203
审稿时长
5.4 months
期刊介绍: Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.
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