Unveiling the cell biology of hippocampal neurons with dendritic axon origin.

IF 7.4 1区 生物学 Q1 CELL BIOLOGY
Journal of Cell Biology Pub Date : 2025-01-06 Epub Date: 2024-11-04 DOI:10.1083/jcb.202403141
Yuhao Han, Daniela Hacker, Bronte Catharina Donders, Christopher Parperis, Roland Thuenauer, Christophe Leterrier, Kay Grünewald, Marina Mikhaylova
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引用次数: 0

Abstract

In mammalian axon-carrying-dendrite (AcD) neurons, the axon emanates from a basal dendrite, instead of the soma, to create a privileged route for action potential generation at the axon initial segment (AIS). However, it is unclear how such unusual morphology is established and whether the structure and function of the AIS in AcD neurons are preserved. By using dissociated hippocampal cultures as a model, we show that the development of AcD morphology can occur prior to synaptogenesis and independently of the in vivo environment. A single precursor neurite first gives rise to the axon and then to the AcD. The AIS possesses a similar cytoskeletal architecture as the soma-derived AIS and similarly functions as a trafficking barrier to retain axon-specific molecular composition. However, it does not undergo homeostatic plasticity, contains lesser cisternal organelles, and receives fewer inhibitory inputs. Our findings reveal insights into AcD neuron biology and underscore AIS structural differences based on axon onset.

揭示具有树突轴突起源的海马神经元的细胞生物学。
在哺乳动物的轴突携带树突(AcD)神经元中,轴突从基部树突而不是从体部发出,从而在轴突起始节段(AIS)为动作电位的产生创造了一条特殊的途径。然而,目前还不清楚这种不寻常的形态是如何形成的,也不清楚AcD神经元的AIS结构和功能是否得以保留。通过使用离体海马培养物作为模型,我们证明 AcD 形态的形成可以发生在突触发生之前,并且不受体内环境的影响。单个前体神经元首先产生轴突,然后产生AcD。AIS具有与源于体细胞的AIS相似的细胞骨架结构,并具有类似的贩运屏障功能,以保留轴突特异性分子组成。但是,它不会发生同态可塑性,含有较少的纤毛细胞器,接受的抑制性输入也较少。我们的研究结果揭示了AcD神经元的生物学特性,并强调了轴突起始阶段的AIS结构差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Cell Biology
Journal of Cell Biology 生物-细胞生物学
CiteScore
12.60
自引率
2.60%
发文量
213
审稿时长
1 months
期刊介绍: The Journal of Cell Biology (JCB) is a comprehensive journal dedicated to publishing original discoveries across all realms of cell biology. We invite papers presenting novel cellular or molecular advancements in various domains of basic cell biology, along with applied cell biology research in diverse systems such as immunology, neurobiology, metabolism, virology, developmental biology, and plant biology. We enthusiastically welcome submissions showcasing significant findings of interest to cell biologists, irrespective of the experimental approach.
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