Role of Orphan ParA Proteins in Replication and Cell Division in Rhodococcus erythropolis PR4.

IF 3.5 4区生物学 Q2 MICROBIOLOGY

Journal of Basic Microbiology Pub Date : 2024-11-03 DOI:10.1002/jobm.202400428

Shabnam Parwin, Preeti Srivastava

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引用次数: 0

Abstract

Bacteria have a very well-regulated mechanism for chromosome segregation and cell division. This process requires a large number of complex proteins to participate and mediate their functionality. Among these complex proteins, ParA and ParB play a vital role for the faithful segregation of chromosome. In Rhodococcus erythropolis PR4, besides the essential parAB operon, there are three orphan copies of parA genes. Here, we report that the orphan ParA2 and ParA3 have distinct roles in the cell cycle. The disruption of the orphan parA2 or parA3 gene resulted in elongated cells. Multiple septal rings and mislocalised septa were observed in ΔparA3 and ΔparA2 mutants, respectively. The subcellular localization of ParA2 revealed a distinct ring- and ribbon-like structure. On the other hand, orphan ParA3 was localized slightly away from the poles. The orphan ParA proteins were found to interact with ParB, the strongest interaction was observed with ParA2. Further, asynchronous replication initiation was observed in ΔparA3 mutants suggesting its role in replication. This is the first report demonstrating the distinct roles of orphan parA genes from Rhodococcus.

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红球菌 PR4 中孤儿 ParA 蛋白在复制和细胞分裂中的作用

细菌的染色体分离和细胞分裂机制非常规范。这一过程需要大量复杂的蛋白质参与并介导其功能。在这些复合蛋白中，ParA 和 ParB 对染色体的忠实分离起着至关重要的作用。在红球菌 PR4 中，除了重要的 ParAB 操作子外，还有三个 ParA 基因的孤儿拷贝。在这里，我们报告了孤儿 ParA2 和 ParA3 在细胞周期中的不同作用。破坏孤儿 parA2 或 parA3 基因会导致细胞变长。在ΔparA3和ΔparA2突变体中分别观察到了多个间隔环和错位的间隔。ParA2 的亚细胞定位显示出明显的环状和带状结构。另一方面，孤儿 ParA3 的定位稍稍偏离两极。研究发现孤儿 ParA 蛋白与 ParB 相互作用，其中与 ParA2 的作用最强。此外，在 ΔparA3 突变体中观察到不同步的复制启动，这表明它在复制中的作用。这是第一份证明 Rhodococcus 的孤儿 parA 基因具有不同作用的报告。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Basic Microbiology 生物-微生物学

CiteScore

6.10

自引率

0.00%

发文量

134

审稿时长

1.8 months

期刊介绍： The Journal of Basic Microbiology (JBM) publishes primary research papers on both procaryotic and eucaryotic microorganisms, including bacteria, archaea, fungi, algae, protozoans, phages, viruses, viroids and prions. Papers published deal with: microbial interactions (pathogenic, mutualistic, environmental), ecology, physiology, genetics and cell biology/development, new methodologies, i.e., new imaging technologies (e.g. video-fluorescence microscopy, modern TEM applications) novel molecular biology methods (e.g. PCR-based gene targeting or cassettes for cloning of GFP constructs).