OsPIPK-FAB, A Negative Regulator in Rice Immunity Unveiled by OsMBL1 Inhibition.

IF 4.8 1区 农林科学 Q1 AGRONOMY
Rice Pub Date : 2024-11-04 DOI:10.1186/s12284-024-00747-3
Ruina Zhang, Mengtian Pei, Shiyi Lin, Jing Chen, Jules Biregeya, Linlin Song, Changlin Peng, Pengcheng Jiang, Guo-Dong Lu
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Abstract

Phosphatidylinositol signaling system plays a crucial role in plant physiology and development, phosphatidylinositol phosphate kinases (PIPKs) are one of the essential enzymes responsible for catalyzing the synthesis of phosphatidylinositol bisphosphate (PIP2) within this signaling pathway. However, its mechanism of signal transduction remains poorly exploited in plants. OsMBL1, a jacalin-related mannose-binding lectin in rice, plays a crucial role in plant defense mechanisms, acting as a key component of the pattern-triggered immunity (PTI) pathway. Here, a rice phosphatidylinositol-phosphate kinase FAB (OsPIPK-FAB), a member of the rice PIPKs family, as an interacting protein of OsMBL1 through yeast-two-hybrid (Y2H) screening assay. And this interaction was confirmed by using co-immunoprecipitation (Co-IP) and pull-down assay techniques. Furthermore, we demonstrated that the deletion of OsPIPK-FAB gene in plant enhanced resistance against rice blast while overexpression of OsPIPK-FAB increases sensitivity to the fungal infection. Additionally, through determination and measurement of the plant inositol 1,4,5-trisphosphate (IP3) contents and the plant phosphatidylinositol 4-phosphate 5-kinase (PIP5K) activity, we revealed that OsMBL1 inhibits the PIP5K kinase activity of OsPIPK-FAB as well as the plant IP3 contents in rice. Conclusively, these findings indicated that OsPIPK-FAB serves as a novel and critical component that is negatively involved in PTI activation and was inhibited by OsMBL1.

通过抑制 OsMBL1 揭示水稻免疫中的负调控因子 OsPIPK-FAB。
磷脂酰肌醇信号系统在植物生理和发育中起着至关重要的作用,磷脂酰肌醇磷酸激酶(PIPKs)是这一信号途径中负责催化磷脂酰肌醇二磷酸(PIP2)合成的重要酶之一。然而,其信号转导机制在植物中的利用率仍然很低。OsMBL1是水稻中一种与贾卡林相关的甘露糖结合凝集素,在植物防御机制中起着至关重要的作用,是模式触发免疫(PTI)途径的关键组成部分。本文通过酵母-双杂交(Y2H)筛选实验发现,水稻磷酸肌醇-磷酸激酶FAB(OsPIPK-FAB)是OsMBL1的互作蛋白。这种相互作用通过共免疫共沉淀(Co-IP)和牵引检测技术得到了证实。此外,我们还证明了在植物体内缺失 OsPIPK-FAB 基因会增强对稻瘟病的抗性,而过表达 OsPIPK-FAB 则会增加对真菌感染的敏感性。此外,通过测定植物体内 1,4,5-三磷酸肌醇(IP3)的含量和植物体内磷脂酰肌醇-4-磷酸 5-激酶(PIP5K)的活性,我们发现 OsMBL1 可抑制 OsPIPK-FAB 的 PIP5K 激酶活性和植物体内 IP3 的含量。这些发现最终表明,OsPIPK-FAB是一种新的关键组分,它负向参与了PTI的激活,并受到OsMBL1的抑制。
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来源期刊
Rice
Rice AGRONOMY-
CiteScore
10.10
自引率
3.60%
发文量
60
审稿时长
>12 weeks
期刊介绍: Rice aims to fill a glaring void in basic and applied plant science journal publishing. This journal is the world''s only high-quality serial publication for reporting current advances in rice genetics, structural and functional genomics, comparative genomics, molecular biology and physiology, molecular breeding and comparative biology. Rice welcomes review articles and original papers in all of the aforementioned areas and serves as the primary source of newly published information for researchers and students in rice and related research.
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