Construction of lysosome-related prognostic signature to predict the survival outcomes and selecting suitable drugs for patients with HNSCC.

IF 5 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
BioFactors Pub Date : 2024-11-04 DOI:10.1002/biof.2140
Bing Cao, Shanshan Gu, Zhisen Shen, Yuna Zhang, Yiming Shen
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引用次数: 0

Abstract

Lysosomes are digestive organelles responsible for endocytosis and autophagy. Recently, the malignancy and invasiveness head and neck squamous cell carcinoma (HNSCC) has been increasingly studied with the role of lysosomes. A list of lysosome-related genes were obtained from MSigDB. A Spearman correlation and univariate Cox regression analyses combined with differential expression analysis were conducted to detect differentially expressed lysosome-related genes related to prognosis. The prediction of prognostic signature was evaluated by plotting survival curve, ROC, and by developing a nomogram. Immune subtypes, infiltration of immune cells, GSVA, TIDE, IC50 of common chemotherapy and targeted therapy, GO, and KEGG function enrichment analyses were carried out to explore the immune microenvironment of the signature. We constructed a lysosome-related prognostic signature that could function as an independent prognostic indicator for patients with HNSCC. High-risk patients were better suited to receive Doxorubicin, Mitomycin C, Pyrimethamine, anti-PD-L1 and anti-CTLA-4 immunotherapy, whereas low-risk patients had sensitivity to Lapatinib. GO functional enrichment analysis showed that prognostic features were strongly associated with epidermis-related functions (e.g., epidermal cell differentiation, epidermal development, and keratinization). In addition, a KEGG function enrichment analysis revealed a potential relationship between the risk assessment model and cardiomyopathy. We constructed a prognostic signature based on lysosome-related genes and successfully predicted the survival outcome of HNSCC patients, which not only provides potential guidance for personalized treatment but also provides a new idea for precision treatment of HNSCC.

构建溶酶体相关预后特征,以预测 HNSCC 患者的生存结果并选择合适的药物。
溶酶体是负责内吞和自噬的消化细胞器。最近,关于头颈部鳞状细胞癌(HNSCC)的恶性程度和侵袭性的研究越来越多地涉及溶酶体的作用。研究人员从 MSigDB 中获得了溶酶体相关基因的列表。通过斯皮尔曼相关分析和单变量考克斯回归分析以及差异表达分析来检测与预后相关的溶酶体相关差异表达基因。通过绘制生存曲线、ROC和建立提名图,对预后特征的预测进行了评估。我们还进行了免疫亚型、免疫细胞浸润、GSVA、TIDE、常见化疗和靶向治疗的IC50、GO和KEGG功能富集分析,以探索特征的免疫微环境。我们构建的溶酶体相关预后特征可作为HNSCC患者的独立预后指标。高危患者更适合接受多柔比星、丝裂霉素C、嘧啶、抗PD-L1和抗CTLA-4免疫疗法,而低危患者对拉帕替尼敏感。GO功能富集分析表明,预后特征与表皮相关功能(如表皮细胞分化、表皮发育和角质化)密切相关。此外,KEGG 功能富集分析显示风险评估模型与心肌病之间存在潜在关系。我们构建了基于溶酶体相关基因的预后特征,并成功预测了HNSCC患者的生存结局,这不仅为个性化治疗提供了潜在指导,也为HNSCC的精准治疗提供了新思路。
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来源期刊
BioFactors
BioFactors 生物-内分泌学与代谢
CiteScore
11.50
自引率
3.30%
发文量
96
审稿时长
6-12 weeks
期刊介绍: BioFactors, a journal of the International Union of Biochemistry and Molecular Biology, is devoted to the rapid publication of highly significant original research articles and reviews in experimental biology in health and disease. The word “biofactors” refers to the many compounds that regulate biological functions. Biological factors comprise many molecules produced or modified by living organisms, and present in many essential systems like the blood, the nervous or immunological systems. A non-exhaustive list of biological factors includes neurotransmitters, cytokines, chemokines, hormones, coagulation factors, transcription factors, signaling molecules, receptor ligands and many more. In the group of biofactors we can accommodate several classical molecules not synthetized in the body such as vitamins, micronutrients or essential trace elements. In keeping with this unified view of biochemistry, BioFactors publishes research dealing with the identification of new substances and the elucidation of their functions at the biophysical, biochemical, cellular and human level as well as studies revealing novel functions of already known biofactors. The journal encourages the submission of studies that use biochemistry, biophysics, cell and molecular biology and/or cell signaling approaches.
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