Cloning, bioinformatics analysis and expression of the cysteine dioxygenase type 1 (CDO1) gene in domestic yak.

IF 2.6 2区农林科学 Q1 VETERINARY SCIENCES

Frontiers in Veterinary Science Pub Date : 2024-10-18 eCollection Date: 2024-01-01 DOI:10.3389/fvets.2024.1488782

Yuxin Fu, Jiuru Yan, Lan Lan, Huizhu Zhang, Peng Wang, Yaying Wang, Xianrong Xiong, Jian Li, Honghong He

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引用次数: 0

Abstract

Introduction: The CDO1 gene is an important gene in the taurine synthesis pathway and has been observed to have high expression in ovaries of female mammals. This study aims to explore the conservation of CDO1 gene in domestic yaks, as well as to examine the fundamental characteristics of CDO1 gene and its expression in female yaks.

Methods: Ovarian samples were collected from yaks in the follicular phase, luteal phase and gestation period in this experiment, and their total RNA and protein were extracted. Then Polymerase Chain Reaction (PCR) and bioinformatics online software were used to clone and analyze the CDO1 gene. The relative expression of CDO1 in yak ovaries was detected by Quantitative Real-time PCR (RT-qPCR) and Western blotting. The distribution and localization of CDO1 protein in ovary were detected by immunohistochemistry.

Results: We have successfully cloned the coding region of CDO1 gene in yak. The results showed that the CDS region of CDO1 gene was 603 bp, encoding 200 amino acids, and was a relatively stable hydrophilic protein. CDO1 is relatively conservative in species evolution. The protein encoded by CDO1 gene does not have a signaling peptide or a transmembrane structure. It is a protein that is not involved in transmembrane transport and is mainly located in the cytoplasm. The secondary structure of the protein is dominated by the random coil. CDO1 is estimated to interact with 10 proteins. The results of RT-qPCR and Western blotting showed that the CDO1 gene exhibited the highest expression in the ovary during the luteal phase and the lowest expression in the ovary during the follicular phase (P < 0.01). The results of immunohistochemistry showed that CDO1 was mainly expressed in granular cells, theca cells and lutein cells of ovarian tissue.

Conclusion: These results suggest that the CDO1 gene has undergone minimal evolutionary changes during the course of animal evolution. The results provide a reference for further investigation of the function of CDO1 gene in reproduction and production in yaks.

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家养牦牛半胱氨酸二氧酶 1 型 (CDO1) 基因的克隆、生物信息学分析和表达。

引言CDO1基因是牛磺酸合成途径中的一个重要基因，在雌性哺乳动物卵巢中具有高表达。本研究旨在探讨 CDO1 基因在家养牦牛中的保存情况，并研究 CDO1 基因的基本特征及其在雌性牦牛中的表达：方法：采集卵泡期、黄体期和妊娠期的牦牛卵巢样本，提取总RNA和蛋白质。然后利用聚合酶链式反应（PCR）和生物信息学在线软件克隆和分析 CDO1 基因。通过实时定量 PCR（RT-qPCR）和 Western 印迹法检测 CDO1 在牦牛卵巢中的相对表达量。免疫组化法检测 CDO1 蛋白在卵巢中的分布和定位：结果：我们成功克隆了牦牛 CDO1 基因的编码区。结果：我们成功克隆了牦牛 CDO1 基因的编码区，结果显示 CDO1 基因的 CDS 区为 603 bp，编码 200 个氨基酸，是一个相对稳定的亲水蛋白。CDO1 在物种进化中相对保守。CDO1 基因编码的蛋白质没有信号肽或跨膜结构。它是一种不参与跨膜运输的蛋白质，主要位于细胞质中。该蛋白质的二级结构以无规线圈为主。CDO1 估计与 10 种蛋白质相互作用。RT-qPCR 和 Western 印迹检测结果显示，CDO1 基因在黄体期卵巢中的表达量最高，而在卵泡期卵巢中的表达量最低（P < 0.01）。免疫组化结果显示，CDO1主要在卵巢组织的颗粒细胞、theca细胞和黄体细胞中表达：结论：这些结果表明 CDO1 基因在动物进化过程中经历了最小的进化变化。这些结果为进一步研究 CDO1 基因在牦牛繁殖和生产中的功能提供了参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Veterinary Science Veterinary-General Veterinary

CiteScore

4.80

自引率

9.40%

发文量

1870

审稿时长

14 weeks

期刊介绍： Frontiers in Veterinary Science is a global, peer-reviewed, Open Access journal that bridges animal and human health, brings a comparative approach to medical and surgical challenges, and advances innovative biotechnology and therapy. Veterinary research today is interdisciplinary, collaborative, and socially relevant, transforming how we understand and investigate animal health and disease. Fundamental research in emerging infectious diseases, predictive genomics, stem cell therapy, and translational modelling is grounded within the integrative social context of public and environmental health, wildlife conservation, novel biomarkers, societal well-being, and cutting-edge clinical practice and specialization. Frontiers in Veterinary Science brings a 21st-century approach—networked, collaborative, and Open Access—to communicate this progress and innovation to both the specialist and to the wider audience of readers in the field. Frontiers in Veterinary Science publishes articles on outstanding discoveries across a wide spectrum of translational, foundational, and clinical research. The journal''s mission is to bring all relevant veterinary sciences together on a single platform with the goal of improving animal and human health.