Post-exercise hot-water immersion is not effective for ribosome biogenesis in rat skeletal muscle.

IF 2.2 3区 医学 Q3 PHYSIOLOGY
Takaya Kotani, Yuki Tamura, Karina Kouzaki, Kazushige Sasaki, Koichi Nakazato
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Abstract

Ribosome biogenesis is an important regulator of skeletal muscle hypertrophy induced by repeated bouts of resistance exercise (RE). Hot-water immersion (HWI), a widely used post-exercise recovery strategy, activates the mechanistic target of rapamycin (mTOR) signaling, a key regulator of ribosome biogenesis in skeletal muscle. However, the effect of HWI on skeletal muscle ribosome biogenesis is not well understood. Here, we aimed to investigate the effects of HWI and post-exercise HWI on ribosome biogenesis using a rat RE model. Male Sprague-Dawley rats were randomly assigned to HWI and non-HWI groups. In both groups, the right leg was isometrically exercised using transcutaneous electrical stimulation, while the left leg was used as an internal non-RE control. Following RE, both limbs were immersed in hot water (41.2 ± 0.03℃) for 20 min under isoflurane anesthesia in the HWI group and the gastrocnemius muscles were sampled at 3 and 24 h post-exercise. HWI significantly increased mTOR signaling and c-Myc mRNA expression, whereas post-exercise HWI significantly increased transcription initiation factor-IA mRNA expression. However, neither HWI nor post-exercise HWI enhanced 45S pre-rRNA expression, ribosomal RNA, or ribosomal protein content. Additionally, HWI tended to decrease 28S rRNA and 18S rRNA content, widely used markers of ribosome content. These results suggest that HWI as a post-exercise recovery is not effective in activating ribosome biogenesis.

运动后热水浸泡对大鼠骨骼肌中核糖体的生物生成无效。
核糖体生物生成是反复阻力运动(RE)诱导骨骼肌肥大的重要调节因素。热水浸泡(HWI)是一种广泛使用的运动后恢复策略,它能激活雷帕霉素机械靶标(mTOR)信号,而mTOR是骨骼肌核糖体生物生成的关键调节因子。然而,HWI 对骨骼肌核糖体生物发生的影响尚不十分清楚。在此,我们旨在利用大鼠 RE 模型研究 HWI 和运动后 HWI 对核糖体生物发生的影响。雄性 Sprague-Dawley 大鼠被随机分配到 HWI 组和非 HWI 组。在这两组中,右腿通过经皮电刺激进行等长运动,而左腿则作为内部非 RE 对照组。RE后,HWI组在异氟烷麻醉下将双肢浸入热水(41.2 ± 0.03℃)中20分钟,并在运动后3小时和24小时采集腓肠肌样本。HWI 能明显增加 mTOR 信号转导和 c-Myc mRNA 的表达,而运动后 HWI 能明显增加转录起始因子-IA mRNA 的表达。然而,HWI 和运动后 HWI 都没有提高 45S pre-rRNA 表达、核糖体 RNA 或核糖体蛋白含量。此外,HWI 有降低 28S rRNA 和 18S rRNA 含量的趋势,而这两种物质是核糖体含量的常用标记。这些结果表明,将 HWI 作为运动后的恢复措施并不能有效激活核糖体的生物生成。
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来源期刊
CiteScore
5.30
自引率
3.60%
发文量
145
审稿时长
2 months
期刊介绍: The American Journal of Physiology-Regulatory, Integrative and Comparative Physiology publishes original investigations that illuminate normal or abnormal regulation and integration of physiological mechanisms at all levels of biological organization, ranging from molecules to humans, including clinical investigations. Major areas of emphasis include regulation in genetically modified animals; model organisms; development and tissue plasticity; neurohumoral control of circulation and hypertension; local control of circulation; cardiac and renal integration; thirst and volume, electrolyte homeostasis; glucose homeostasis and energy balance; appetite and obesity; inflammation and cytokines; integrative physiology of pregnancy-parturition-lactation; and thermoregulation and adaptations to exercise and environmental stress.
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