Structure analysis and immunomodulatory activity of novel oligosaccharide from Nicotiana tabacum roots

IF 2.4 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research Pub Date : 2024-11-01 DOI:10.1016/j.carres.2024.109303

引用次数: 0

Abstract

A novel oligosaccharide (NTRP60-W-2) with an average molecular weight of 1377 Da was isolated and purified from Nicotiana tabacum roots. Its structural characteristics and immunomodulatory properties were investigated. Structural analysis revealed that NTRP60-W-2 was composed exclusively of glucose, featuring →1)-α-D-Glcp-(6→ backbone. Immunological assays demonstrated that NTRP60-W-2 significantly enhanced cell viability, nitric oxide production and cytokine secretion (IL-6 and TNF-α) in RAW264.7 cells. These findings provide a foundation for further exploration of Nicotiana tabacum carbohydrates and their potential biological activities.

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来自烟草根的新型寡糖的结构分析和免疫调节活性。

从烟草根中分离纯化了一种新型寡糖（NTRP60-W-2），其平均分子量为 1377 Da。研究了其结构特征和免疫调节特性。结构分析表明，NTRP60-W-2完全由葡萄糖组成，具有→1)-α-D-Glcp-(6→骨架。免疫学试验表明，NTRP60-W-2 能显著提高 RAW264.7 细胞的存活率、一氧化氮产量和细胞因子分泌（IL-6 和 TNF-α）。这些发现为进一步探索烟草碳水化合物及其潜在的生物活性奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Carbohydrate Research 化学-生化与分子生物学

CiteScore

5.00

自引率

3.20%

发文量

183

审稿时长

3.6 weeks

期刊介绍： Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".