Does Fusobacterium in Colorectal Cancer Sites Originate From the Oral Cavity? A Pilot Study

IF 1.7 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research Pub Date : 2024-11-03 DOI:10.1002/cre2.70016

Niels Plomp, Kristina Bertl, Marie-Louise Lydrup, Klas Sjöberg, Hermie J. M. Harmsen, Andreas Stavropoulos

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引用次数: 0

Abstract

Objectives

Fusobacterium can contribute to oral diseases, but also pose as a systemic risk factor. This genus, and especially F. nucleatum, can be found in colorectal cancer (CRC) tissue and is involved in multiple aspects of this type of cancer. Previous studies indicated a possible oral origin of these bacteria; however, stronger evidence is needed to reach a definitive conclusion. This pilot study aimed to establish a method to successfully compare, at the strain level, fusobacteria from the oral cavity and CRC resection material for future cohort studies of CRC patients.

Material and Methods

In a first cohort of eight periodontitis patients, gingival crevicular fluid and saliva were collected. Fusobacterium was isolated on two different media. In a second cohort, saliva and CRC resection material were collected from ten CRC patients. These samples were used for screening of Fusobacterium with culturing, 16S rRNA gene profiling and a PCR-based approach.

Results

In the first cohort, different Fusobacterium species were identified in GCF and saliva samples. However, as the total yield of Fusobacterium seemed slightly higher in saliva samples, it was therefore preferred for subsequent sample collection. Thus, in the second cohort, patient-matched saliva and CRC resection material were screened for Fusobacterium and this showed that nine patients were culture-positive in the saliva samples; however, no Fusobacterium could be isolated from the resection material. On the other hand, 16S rRNA gene profiling of the resection material indicated that eight CRC patients were positive for Fusobacterium. All eight of these patients carried Fusobacterium in their saliva, indicated by both marker gene PCR and culture-based screening.

Conclusions

These pilot results are compatible with data from previous studies, indicating a possible link between oral and CRC-associated Fusobacterium, and a more in-depth analysis of specific strains and their characteristics in a larger cohort is justified.

Trial Registration

The protocol was registered at clinicaltrials.gov (NCT05945082).

Abstract Image

查看原文本刊更多论文

大肠癌病灶中的镰刀菌是否来自口腔？一项试点研究。

目的：镰刀菌可导致口腔疾病，但也是一种全身性风险因素。在结肠直肠癌（CRC）组织中可发现该菌属，尤其是核酸镰刀菌，并与此类癌症的多个方面有关。以前的研究表明，这些细菌可能来源于口腔，但要得出确切的结论还需要更有力的证据。这项试验性研究旨在建立一种方法，成功地在菌株水平上比较口腔和 CRC 切除材料中的镰刀菌，以便将来对 CRC 患者进行队列研究：材料：在第一批 8 名牙周炎患者中收集了牙龈缝隙液和唾液。在两种不同的培养基上分离出镰刀菌。在第二组样本中，收集了 10 名乳腺癌患者的唾液和乳腺癌切除材料。通过培养、16S rRNA 基因图谱分析和基于 PCR 的方法对这些样本进行了镰刀菌筛选：结果：在第一组样本中，GCF 和唾液样本中发现了不同的镰刀菌种类。然而，由于唾液样本中的镰刀菌总产量似乎略高，因此在随后的样本采集中更倾向于唾液样本。因此，在第二个队列中，对与患者匹配的唾液和 CRC 切片材料进行了镰刀菌筛查，结果显示，9 名患者的唾液样本培养呈阳性；但从切除材料中无法分离出镰刀菌。另一方面，对切除材料进行的 16S rRNA 基因分析表明，8 名 CRC 患者的镰刀菌阳性。通过标记基因 PCR 和培养基筛查，这八名患者的唾液中均含有镰刀菌：这些试验结果与之前的研究数据相符，表明口腔和 CRC 相关镰刀菌之间可能存在联系，因此有必要在更大的群体中对特定菌株及其特征进行更深入的分析：试验方案已在 clinicaltrials.gov (NCT05945082) 上注册。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical and Experimental Dental Research DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.30

自引率

5.60%

发文量

165

审稿时长

26 weeks

期刊介绍： Clinical and Experimental Dental Research aims to provide open access peer-reviewed publications of high scientific quality representing original clinical, diagnostic or experimental work within all disciplines and fields of oral medicine and dentistry. The scope of Clinical and Experimental Dental Research comprises original research material on the anatomy, physiology and pathology of oro-facial, oro-pharyngeal and maxillofacial tissues, and functions and dysfunctions within the stomatognathic system, and the epidemiology, aetiology, prevention, diagnosis, prognosis and therapy of diseases and conditions that have an effect on the homeostasis of the mouth, jaws, and closely associated structures, as well as the healing and regeneration and the clinical aspects of replacement of hard and soft tissues with biomaterials, and the rehabilitation of stomatognathic functions. Studies that bring new knowledge on how to advance health on the individual or public health levels, including interactions between oral and general health and ill-health are welcome.