Regulation of melanization in aphids by parasitoid wasp venom proteins enhances mummification.

IF 3.8 1区农林科学 Q1 AGRONOMY

Pest Management Science Pub Date : 2024-11-04 DOI:10.1002/ps.8503

Jin Zhao, Zheng-Wu Wang, Guangmao Shen, Die Hu, Yi Zhong, Chao Ye, Jin-Jun Wang

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引用次数: 0

Abstract

Background: Interactions between parasitic insects and their hosts demonstrate the complexity of evolutionary processes. Specifically, the parasitoid wasp Aphidius ervi manipulates its host, the pea aphid Acyrthosiphon pisum, through strategic venom injection to enhance mummification. This study explores how this venom affects the aphid's immune system, particularly targeting the activity of the phenoloxidase (PO) enzyme.

Results: Following the injection of venom from A. ervi, significant changes were observed in the expression of immune-related genes in A. pisum, especially notable expression changes of ApPPOs and a reduction of PO activity. Multi-omics sequencing identified 74 potential venom proteins in the venom gland of A. ervi, including serine protease homolog 1 (AeSPH1) and serine protease inhibitor (AeSPN1), hypothesized to regulate PO activity. The injection of recombinant protein AeSPH1 and AeSPN1 into the A. pisum hemocoel selectively reduced the expression of ApPPO1, without affecting ApPPO2, and effectively suppressed melanization. Moreover, RNAi targeting AeSPH1 significantly reduced the mummification rate in A. pisum population parasitized by A. ervi.

Conclusion: Our findings clarify the complex biochemical mechanisms underlying host-wasp interactions and highlight potential avenues for developing targeted biological control strategies. © 2024 Society of Chemical Industry.

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寄生蜂毒液蛋白对蚜虫黑色素化的调控可促进木乃伊化。

背景：寄生昆虫与其宿主之间的相互作用显示了进化过程的复杂性。具体来说，寄生蜂蚜茧蜂（Aphidius ervi）通过有策略地注射毒液来操纵宿主豌豆蚜（Acyrthosiphon pisum），以增强其木乃伊化。本研究探讨了这种毒液如何影响蚜虫的免疫系统，特别是针对酚氧化酶（PO）的活性：结果：注射 A. ervi 毒液后，观察到 A. pisum 免疫相关基因的表达发生了显著变化，尤其是 ApPPOs 的表达发生了明显变化，PO 活性降低。多组学测序确定了 A. ervi 毒腺中 74 种潜在的毒液蛋白，包括丝氨酸蛋白酶同源物 1（AeSPH1）和丝氨酸蛋白酶抑制物（AeSPN1），据推测这两种蛋白可调节 PO 活性。将重组蛋白 AeSPH1 和 AeSPN1 注入 A. pisum 血肠，可选择性地减少 ApPPO1 的表达，而不影响 ApPPO2 的表达，并有效抑制黑色素形成。此外，针对 AeSPH1 的 RNAi 能显著降低被 A. ervi 寄生的 A. pisum 群体的木乃伊化率：我们的研究结果阐明了寄主与黄蜂相互作用的复杂生化机制，并强调了开发有针对性的生物防治策略的潜在途径。© 2024 化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.