{"title":"Transgenerational response of glucose metabolism in Caenorhabditis elegans exposed to 6-PPD quinone","authors":"Zhenjun Liu , Yunhui Li , Dayong Wang","doi":"10.1016/j.chemosphere.2024.143653","DOIUrl":null,"url":null,"abstract":"<div><div>In <em>Caenorhabditis elegans</em>, 6-PPD quinine (6-PPDQ) could cause several aspects of toxicity together with alteration in glucose metabolism. However, transgenerational alteration in glucose metabolism remains still unknown after 6-PPDQ exposure. In the current study, we further observed transgenerational increase in glucose content induced by 6-PPDQ (1–10 μg/L). After 1–10 μg/L 6-PPDQ exposure, although expressions of genes controlling gluconeogenesis were not changed in the offspring, expressions of <em>hxk-1</em>, <em>hxk-3</em>, <em>pyk-1</em>, and <em>pyk-2</em> controlling glycolysis could be decreased in the offspring. Meanwhile, transgenerational decrease in expressions of <em>daf-16</em> encoding FOXO transcriptional factor and <em>aak-2</em> encoding AMPK was detected in the offspring of 6-PPDQ (1–10 μg/L) exposed nematodes. RNAi of <em>daf-16</em> and <em>aak-2</em> led to more severe transgenerational increase in glucose content and reduction in expressions of <em>hxk-1</em> and <em>hxk-3</em> induced by 6-PPDQ. Moreover, RNAi of <em>daf-16</em>, <em>aak-2</em>, <em>hxk-1</em>, <em>hxk-3</em>, <em>pyk-1</em>, and <em>pyk-2</em> caused susceptibility to transgenerational 6-PPDQ toxicity on locomotion and reproduction. Additionally, 6-PPDQ induced activation of SOD-3 and HSP-6 reflecting anti-oxidation and mitochondrial UPR responses could be inhibited by RNAi of <em>daf-16</em>, <em>aak-2</em>, <em>hxk-1</em>, <em>hxk-3</em>, <em>pyk-1</em>, and <em>pyk-2</em>. Therefore, exposure to 6-PPDQ potentially resulted in transgenerational alteration in glucose metabolism, which provided the possible link to induction of transgenerational 6-PPDQ toxicity in organisms.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":null,"pages":null},"PeriodicalIF":8.1000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemosphere","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0045653524025530","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
In Caenorhabditis elegans, 6-PPD quinine (6-PPDQ) could cause several aspects of toxicity together with alteration in glucose metabolism. However, transgenerational alteration in glucose metabolism remains still unknown after 6-PPDQ exposure. In the current study, we further observed transgenerational increase in glucose content induced by 6-PPDQ (1–10 μg/L). After 1–10 μg/L 6-PPDQ exposure, although expressions of genes controlling gluconeogenesis were not changed in the offspring, expressions of hxk-1, hxk-3, pyk-1, and pyk-2 controlling glycolysis could be decreased in the offspring. Meanwhile, transgenerational decrease in expressions of daf-16 encoding FOXO transcriptional factor and aak-2 encoding AMPK was detected in the offspring of 6-PPDQ (1–10 μg/L) exposed nematodes. RNAi of daf-16 and aak-2 led to more severe transgenerational increase in glucose content and reduction in expressions of hxk-1 and hxk-3 induced by 6-PPDQ. Moreover, RNAi of daf-16, aak-2, hxk-1, hxk-3, pyk-1, and pyk-2 caused susceptibility to transgenerational 6-PPDQ toxicity on locomotion and reproduction. Additionally, 6-PPDQ induced activation of SOD-3 and HSP-6 reflecting anti-oxidation and mitochondrial UPR responses could be inhibited by RNAi of daf-16, aak-2, hxk-1, hxk-3, pyk-1, and pyk-2. Therefore, exposure to 6-PPDQ potentially resulted in transgenerational alteration in glucose metabolism, which provided the possible link to induction of transgenerational 6-PPDQ toxicity in organisms.
期刊介绍:
Chemosphere, being an international multidisciplinary journal, is dedicated to publishing original communications and review articles on chemicals in the environment. The scope covers a wide range of topics, including the identification, quantification, behavior, fate, toxicology, treatment, and remediation of chemicals in the bio-, hydro-, litho-, and atmosphere, ensuring the broad dissemination of research in this field.