Transgenerational response of glucose metabolism in Caenorhabditis elegans exposed to 6-PPD quinone

IF 8.1 2区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES
Zhenjun Liu , Yunhui Li , Dayong Wang
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引用次数: 0

Abstract

In Caenorhabditis elegans, 6-PPD quinine (6-PPDQ) could cause several aspects of toxicity together with alteration in glucose metabolism. However, transgenerational alteration in glucose metabolism remains still unknown after 6-PPDQ exposure. In the current study, we further observed transgenerational increase in glucose content induced by 6-PPDQ (1–10 μg/L). After 1–10 μg/L 6-PPDQ exposure, although expressions of genes controlling gluconeogenesis were not changed in the offspring, expressions of hxk-1, hxk-3, pyk-1, and pyk-2 controlling glycolysis could be decreased in the offspring. Meanwhile, transgenerational decrease in expressions of daf-16 encoding FOXO transcriptional factor and aak-2 encoding AMPK was detected in the offspring of 6-PPDQ (1–10 μg/L) exposed nematodes. RNAi of daf-16 and aak-2 led to more severe transgenerational increase in glucose content and reduction in expressions of hxk-1 and hxk-3 induced by 6-PPDQ. Moreover, RNAi of daf-16, aak-2, hxk-1, hxk-3, pyk-1, and pyk-2 caused susceptibility to transgenerational 6-PPDQ toxicity on locomotion and reproduction. Additionally, 6-PPDQ induced activation of SOD-3 and HSP-6 reflecting anti-oxidation and mitochondrial UPR responses could be inhibited by RNAi of daf-16, aak-2, hxk-1, hxk-3, pyk-1, and pyk-2. Therefore, exposure to 6-PPDQ potentially resulted in transgenerational alteration in glucose metabolism, which provided the possible link to induction of transgenerational 6-PPDQ toxicity in organisms.

Abstract Image

暴露于 6-PPD 醌的秀丽隐杆线虫葡萄糖代谢的代际反应。
在秀丽隐杆线虫体内,6-PPD 奎宁(6-PPDQ)可导致多方面的毒性,同时改变葡萄糖代谢。然而,6-PPDQ 暴露后葡萄糖代谢的跨代改变仍是一个未知数。在本研究中,我们进一步观察了 6-PPDQ(1-10 μg/L)诱导的葡萄糖含量的代际增加。在暴露于 1-10 μg/L 6-PPDQ 后,虽然控制葡萄糖生成的基因在后代中的表达没有变化,但控制糖酵解的 hxk-1、hxk-3、pyk-1 和 pyk-2 在后代中的表达可能会降低。同时,在暴露于 6-PPDQ(1-10 μg/L)的线虫后代中,发现编码 FOXO 转录因子的 daf-16 和编码 AMPK 的 aak-2 的表达量出现了转代性下降。对 daf-16 和 aak-2 进行 RNAi 后,6-PPDQ 诱导的葡萄糖含量和 hxk-1 和 hxk-3 的表达量会出现更严重的转代增加。此外,daf-16、aak-2、hxk-1、hxk-3、pyk-1 和 pyk-2 的 RNAi 会导致 6-PPDQ 对运动和繁殖的转代毒性。此外,通过对daf-16、aak-2、hxk-1、hxk-3、pyk-1和pyk-2进行RNAi,可降低6-PPDQ诱导的SOD-3和HSP-6的活化,这反映了抗氧化和线粒体UPR反应。因此,暴露于 6-PPDQ 可能会导致葡萄糖代谢的跨代改变,这为诱导生物体内 6-PPDQ 的跨代毒性提供了可能的联系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Chemosphere
Chemosphere 环境科学-环境科学
CiteScore
15.80
自引率
8.00%
发文量
4975
审稿时长
3.4 months
期刊介绍: Chemosphere, being an international multidisciplinary journal, is dedicated to publishing original communications and review articles on chemicals in the environment. The scope covers a wide range of topics, including the identification, quantification, behavior, fate, toxicology, treatment, and remediation of chemicals in the bio-, hydro-, litho-, and atmosphere, ensuring the broad dissemination of research in this field.
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