Transcriptomic evidence of black soybean ethanolic extract and 2-aminobutyric acid in suppressing neuroinflammation and enhancing synaptic transmission

IF 6.9 2区医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL

Biomedicine & Pharmacotherapy Pub Date : 2024-11-01 DOI:10.1016/j.biopha.2024.117633

Sharmin Aktar , Ayumi Kato , Kyoko Toda , Shinya Takahashi , Mari Maeda-Yamamoto , Farhana Ferdousi , Hiroko Isoda

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引用次数: 0

Abstract

Introduction

Recently, the awareness of the beneficial utilization of natural bioactive compounds in treating neuroinflammation has gained particular attention. We aimed to understand the anti-neuroinflammatory effect of black soybeans (Glycine max (L.) Merr) ethanolic extract (BBEE) and its bioactive compound, 2-aminobutyric acid (2-AB), against LPS-induced SH-SY5Y cells.

Method

Cell viability and the optimum therapeutic dose were confirmed by MTT assay. We conducted a whole-transcriptomic analysis of BBEE and 2-AB in LPS-induced SH-SY5Y cells using microarray normalized with SST-RMA. DEGs were selected based on p-value < 0.05 and fold change > 2, and validated by RT-qPCR and immunocytochemical analyses.

Results

We found that both BBEE and 2-AB down-regulated the expression of inflammatory cytokines IL6 and TNFA under LPS-induced conditions. This was also observed in the microarray data, showing downregulation of several inflammatory pathways, such as NF-kB, and IL6-JAK/STAT3-signaling pathways. In contrast, it upregulated the expression of CALML3, GRIN2, and GRIA2 gene expressions, which influence the AMPK and CAMK2 signaling pathways, indicating the potential of BBEE in neurotransmission and synaptic function. Also, immunofluorescence analysis revealed that 2-AB treatment significantly increased PSD-95 and Ca²⁺ levels, suggesting its effect on synaptic transmission essential for brain function.

Conclusion

Our findings suggest the potential anti-neuroinflammatory effects of BBEE and 2-AB, which may offer therapeutic and preventive benefits in mitigating neurological disorders. Given that BB is widely consumed in many Asian countries, our study may encourage its incorporation into the daily diet to slow inflammation-induced neurodegenerative disorders, reduce age-related cognitive decline, and enhance overall brain function.

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黑大豆乙醇提取物和 2-氨基丁酸抑制神经炎症和增强突触传递的转录组学证据

简介最近，人们意识到利用天然生物活性化合物治疗神经炎症是有益的，并对此给予了特别关注。我们旨在了解黑大豆（Glycine max (L.) Merr）乙醇提取物（BBEE）及其生物活性化合物 2-氨基丁酸（2-AB）对 LPS 诱导的 SH-SY5Y 细胞的抗神经炎症作用：方法：细胞活力和最佳治疗剂量由 MTT 试验确定。我们用 SST-RMA 归一化的芯片对 BBEE 和 2-AB 在 LPS 诱导的 SH-SY5Y 细胞中的作用进行了全转录组分析。根据 p 值 < 0.05 和折叠变化 > 2 筛选 DEGs，并通过 RT-qPCR 和免疫细胞化学分析进行验证：结果：我们发现，在 LPS 诱导的条件下，BBEE 和 2-AB 都能下调炎性细胞因子 IL6 和 TNFA 的表达。微阵列数据也观察到了这一点，显示了几种炎症通路的下调，如 NF-kB、IL6-JAK/STAT3 信号通路。相反，它却能上调影响 AMPK 和 CAMK2 信号通路的 CALML3、GRIN2 和 GRIA2 基因表达，这表明 BBEE 在神经传递和突触功能方面具有潜力。此外，免疫荧光分析表明，2-AB 处理可显著提高 PSD-95 和 Ca2+ 水平，表明其对大脑功能所必需的突触传递有影响：我们的研究结果表明，BBEE和2-AB具有潜在的抗神经炎症作用，可为缓解神经系统疾病提供治疗和预防益处。鉴于 BB 在许多亚洲国家被广泛食用，我们的研究可能会鼓励将其纳入日常饮食，以减缓炎症引起的神经退行性疾病，减少与年龄相关的认知能力下降，并增强大脑的整体功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomedicine & Pharmacotherapy 医学-药学

CiteScore

11.90

自引率

2.70%

发文量

1621

审稿时长

48 days

期刊介绍： Biomedicine & Pharmacotherapy stands as a multidisciplinary journal, presenting a spectrum of original research reports, reviews, and communications in the realms of clinical and basic medicine, as well as pharmacology. The journal spans various fields, including Cancer, Nutriceutics, Neurodegenerative, Cardiac, and Infectious Diseases.