Second generation multiple channeling using platelet-rich plasma enhances cartilage repair through recruitment of endogenous MSCs in bone marrow.

IF 5.4 2区 医学 Q1 CELL & TISSUE ENGINEERING
Min Ji Lee, Jian Jiang, Soo Hyun Kim, Chris Hyunchul Jo
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Abstract

In the treatment of cartilage defects, a key factor is the adequate and specific recruitment of endogenous stem cells to the site of injury. However, the limited quantity and capability of endogenous bone marrow stem cells (BM MSCs) often result in the formation of fibrocartilage when using bone marrow stimulation (BMS) procedures. We engineered second-generation platelet-rich plasma (2G PRP) with defibrinogenating and antifibrinolytic agents for injection into the condyle of the right femur, followed by multiple channeling (MCh) 5 days later. This approach aims to enhance repair by promoting the local proliferation and migration of BM MSCs to the full-thickness knee cartilage defect (ftKD). In our in vitro study, 2G PRP increased the number of endogenous BM MSCs and their ability to migrate toward an IL-1β-induced inflammatory condition. This significance was further confirmed by in vivo proliferation results after injection of 2G PRP into the condyle of rats. Fifty-four healthy male Sprague-Dawley rats were divided into 3 groups (ftKD, MCh, 2G MCh) for 3 time points (2 weeks, 4 weeks, 8 weeks). The 2G MCh (2G PRP injection + MCh) groups significantly improved cartilage formation at 4 and 8 weeks compared to the ftKD and MCh groups. The 2G MCh initiated cartilage repair earlier than MCh and significantly enhanced up to 8 weeks. This study demonstrated that 2G PRP increased the number of BM MSCs through the enhancement of proliferation and recruitment into the injured site, thereby improving articular cartilage repair.

使用富血小板血浆的第二代多通道疗法通过招募骨髓中的内源性间充质干细胞增强软骨修复。
在软骨缺损的治疗中,一个关键因素是将内源性干细胞充分而有针对性地招募到损伤部位。然而,由于内源性骨髓干细胞(BM MSCs)的数量和能力有限,在使用骨髓刺激(BMS)程序时,往往会导致纤维软骨的形成。我们在第二代富血小板血浆(2G PRP)中加入了去纤维蛋白原化剂和抗纤维蛋白溶解剂,将其注射到右股骨髁部,5天后再进行多通道注射(MCh)。这种方法旨在通过促进骨髓间充质干细胞向全厚膝关节软骨缺损(ftKD)的局部增殖和迁移来加强修复。在我们的体外研究中,2G PRP 增加了内源性 BM 间充质干细胞的数量及其向 IL-1β 诱导的炎症条件下迁移的能力。向大鼠髁突注射 2G PRP 后的体内增殖结果进一步证实了这一意义。54只健康的雄性Sprague-Dawley大鼠被分为3组(ftKD组、MCh组、2G MCh组),共3个时间点(2周、4周、8周)。与ftKD组和MCh组相比,2G MCh组(2G PRP注射+MCh)在4周和8周时明显改善了软骨的形成。2G MCh 比 MCh 更早启动软骨修复,并在 8 周内明显增强。这项研究表明,2G PRP 可通过促进增殖和招募进入损伤部位来增加 BM 间充质干细胞的数量,从而改善关节软骨修复。
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来源期刊
Stem Cells Translational Medicine
Stem Cells Translational Medicine CELL & TISSUE ENGINEERING-
CiteScore
12.90
自引率
3.30%
发文量
140
审稿时长
6-12 weeks
期刊介绍: STEM CELLS Translational Medicine is a monthly, peer-reviewed, largely online, open access journal. STEM CELLS Translational Medicine works to advance the utilization of cells for clinical therapy. By bridging stem cell molecular and biological research and helping speed translations of emerging lab discoveries into clinical trials, STEM CELLS Translational Medicine will help move applications of these critical investigations closer to accepted best patient practices and ultimately improve outcomes. The journal encourages original research articles and concise reviews describing laboratory investigations of stem cells, including their characterization and manipulation, and the translation of their clinical aspects of from the bench to patient care. STEM CELLS Translational Medicine covers all aspects of translational cell studies, including bench research, first-in-human case studies, and relevant clinical trials.
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