Separation of nucleobases, nucleosides, nucleotides and oligonucleotides by hydrophilic interaction liquid chromatography (HILIC): A state-of-the-art review

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography A Pub Date : 2024-10-22 DOI:10.1016/j.chroma.2024.465467

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引用次数: 0

Abstract

The polar nature of nucleobases, nucleosides and nucleotides makes hydrophilic interaction chromatography (HILIC) a good choice of technology for separation. Both naturally occurring and modified nucleosides and nucleotides have been successfully separated in HILIC. A wide range of stationary phases with different retention and selectivity are suitable for the separation of nucleobases, nucleosides and nucleotides; and a sufficient knowledge base is also available to guide method development. Although oligonucleotides are significantly different from nucleotides in terms of polarity and charges, HILIC has been shown to be a viable alternative to ion-pairing reversed-phase liquid chromatography (IP-RPLC). Only a few polar stationary phases have been shown to provide satisfactory performance; however, the requirements for the mobile phase composition including organic solvent, mobile phase pH and salt concentration are sufficiently understood. This review provides a comprehensive evaluation of the chromatographic conditions with a historical perspective on adopting and developing HILIC for the separation of nucleobases, nucleosides, nucleotides and oligonucleotides. The areas for more research and potential directions for future development activities are identified and discussed.

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亲水作用液相色谱法（HILIC）分离核碱基、核苷、核苷酸和寡核苷酸：最新进展综述。

核碱基、核苷和核苷酸的极性使亲水相互作用色谱（HILIC）成为分离技术的最佳选择。天然核苷和核苷酸以及经过修饰的核苷和核苷酸均已在 HILIC 中成功分离。具有不同保留和选择性的多种固定相都适用于核碱基、核苷和核苷酸的分离，而且有足够的知识基础来指导方法的开发。虽然寡核苷酸在极性和电荷方面与核苷酸有很大不同，但 HILIC 已被证明是离子配对反相液相色谱（IP-RPLC）的可行替代方法。只有少数极性固定相能提供令人满意的性能；不过，人们对流动相组成（包括有机溶剂、流动相 pH 值和盐浓度）的要求已经有了充分的了解。本综述从采用和发展 HILIC 分离核酸、核苷、核苷酸和寡核苷酸的历史角度，对色谱条件进行了全面评估。本文还确定并讨论了需要进一步研究的领域以及未来开发活动的潜在方向。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Chromatography A 化学-分析化学

CiteScore

7.90

自引率

14.60%

发文量

742

审稿时长

45 days

期刊介绍： The Journal of Chromatography A provides a forum for the publication of original research and critical reviews on all aspects of fundamental and applied separation science. The scope of the journal includes chromatography and related techniques, electromigration techniques (e.g. electrophoresis, electrochromatography), hyphenated and other multi-dimensional techniques, sample preparation, and detection methods such as mass spectrometry. Contributions consist mainly of research papers dealing with the theory of separation methods, instrumental developments and analytical and preparative applications of general interest.