Limonene encapsulated alginate/collagen as antibiofilm drug against Acinetobacter baumannii.

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Fatemeh-Sadat GholamhosseinTabar Valookolaei, Hossein Sazegar, Leila Rouhi
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引用次数: 0

Abstract

This work examined the antibacterial and antibiofilm properties of alginate/collagen nanoparticles containing limonene. The multi-drug resistant (MDR) strains were screened, and the morphological features of the produced nanoparticles were determined utilizing SEM, DLS, and FTIR. Additionally, the encapsulation effectiveness, stability, and drug release were assessed. The levels of OmpA and Bap biofilm genes were assessed using qRT-PCR. At the same time, the antibacterial and cytotoxic activities of the nanoparticles were evaluated using well diffusion and MTT techniques, respectively. LAC nanoparticles measuring 300 ± 9.6 nm in size, 83.64 ± 0.19% encapsulation efficiency, and 60-day stability at 4 °C were synthesized. The biological investigation demonstrated that LAC nanoparticles had potent antibacterial capabilities. This was shown by their ability to significantly decrease the transcription of OmpA and Bap biofilm genes at a statistically significant level of p ≤ 0.05. The nanoparticles exhibited reduced antibiotic resistance compared to free limonene and alginate/collagen. Compared to limonene, LAC nanoparticles exhibited negligible cytotoxicity against HEK-293 at doses ranging from 1.56 to 100 µg/mL (p ≤ 0.01). The findings underscore the potential of LAC nanoparticles as a breakthrough in the fight against highly resistant pathogens. The potent antibacterial effects of LAC nanoparticles versus Acinetobacter baumannii (A. baumannii) MDR strains, considered highly resistant pathogens of significant concern, could inspire new strategies in antibacterial research.

将柠檬烯包裹的海藻酸盐/胶原蛋白作为抗鲍曼不动杆菌的生物膜药物。
本研究考察了含有柠檬烯的海藻酸/胶原蛋白纳米粒子的抗菌和抗生物膜特性。筛选了耐多药(MDR)菌株,并利用扫描电镜、DLS 和傅立叶变换红外光谱测定了所制纳米粒子的形态特征。此外,还对封装效果、稳定性和药物释放进行了评估。利用 qRT-PCR 技术评估了 OmpA 和 Bap 生物膜基因的水平。同时,还分别使用井扩散和 MTT 技术评估了纳米颗粒的抗菌和细胞毒性活性。合成的 LAC 纳米粒子大小为 300 ± 9.6 nm,封装效率为 83.64 ± 0.19%,在 4 °C 下可稳定 60 天。生物学研究表明,LAC 纳米粒子具有强大的抗菌能力。这表现在它们能够显著降低 OmpA 和 Bap 生物膜基因的转录,且 p ≤ 0.05 具有统计学意义。与游离的柠檬烯和海藻酸/胶原相比,纳米粒子表现出更低的抗生素耐药性。与柠檬烯相比,在剂量为 1.56 至 100 µg/mL 时,LAC 纳米粒子对 HEK-293 的细胞毒性可忽略不计(p ≤ 0.01)。这些发现凸显了 LAC 纳米粒子在抗击高抗药性病原体方面的突破潜力。LAC 纳米粒子对鲍曼不动杆菌(A. baumannii)MDR 菌株的强效抗菌作用可激发抗菌研究的新策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Biotechnology
BMC Biotechnology 工程技术-生物工程与应用微生物
CiteScore
6.60
自引率
0.00%
发文量
34
审稿时长
2 months
期刊介绍: BMC Biotechnology is an open access, peer-reviewed journal that considers articles on the manipulation of biological macromolecules or organisms for use in experimental procedures, cellular and tissue engineering or in the pharmaceutical, agricultural biotechnology and allied industries.
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