Effects of phosphorylation on CsTT12 transport function: A comparative phosphoproteomic analysis of flavonoid biosynthesis in tea plants (Camellia sinensis).

IF 6.2 1区 生物学 Q1 PLANT SCIENCES
Na-Na Wang, Ke-Yan Xiu, Min Deng, Qi-Yun Liu, Di-Di Jin, Qiao-Mei Zhao, Huang-Qiang Su, Ting-Ting Qiu, Hai-Yan Wang, Ya-Jun Liu, Xiao-Lan Jiang, Tao Xia, Li-Ping Gao
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Abstract

Monomeric flavan-3-ols and their oligomeric forms, proanthocyanidins (PAs), are closely related to the bitterness of tea beverages. Monomeric flavan-3-ols are characteristic flavor compounds in tea. Increasing the content of PAs and anthocyanins enhances the resistance of tea plants to pathogen invasion but decreases the quality of tea beverages. MATE family transporters play a critical role in transferring monomeric flavan-3-ols and anthocyanins into vacuoles for storage or subsequent condensation into PAs. Their activities modulate the ratio of monomeric flavan-3-ols to PAs and increase anthocyanin content in tea plants. In this study, it was observed that the gene expression and protein phosphorylation level of the MATE transporter CsTT12, a vacuole-localized flavonoid transporter, were notably upregulated following exogenous sucrose treatment, promoting PA synthesis in tea plants. Further analysis revealed that overexpression of CsTT12 and CsTT12S17D significantly increased the content of anthocyanins and PAs in plants, whereas CsTT12S17A did not. In CsTT12 knockdown plants, PA's accumulation decreased significantly, while monomeric catechin content increased. Moreover, phosphorylation modification enhanced the vacuolar membrane localization of CsTT12, whereas dephosphorylation weakened its vacuolar membrane localization. This study uncovers the crucial role of phosphorylation in flavonoid biosynthesis and provides insights into balancing quality improvements and resistance enhancement.

磷酸化对 CsTT12 转运功能的影响:茶树(Camellia sinensis)黄酮类化合物生物合成的磷酸化蛋白质组比较分析
单体黄烷-3-醇及其低聚体形式原花青素(PA)与茶饮料的苦味密切相关。单体黄烷-3-醇是茶叶中特有的风味化合物。增加 PAs 和花青素的含量可增强茶树抵抗病原体入侵的能力,但会降低茶饮料的品质。MATE 家族转运体在将单体黄烷-3-醇和花青素转移到液泡中储存或随后缩合成 PA 方面发挥着关键作用。它们的活动调节了单体黄烷-3-醇与 PA 的比例,增加了茶树中的花青素含量。本研究观察到,外源蔗糖处理后,液泡定位的黄酮类转运体MATE转运体CsTT12的基因表达和蛋白磷酸化水平显著上调,促进了茶树中PA的合成。进一步的分析表明,过表达 CsTT12 和 CsTT12S17D 能显著增加植物中花青素和 PA 的含量,而 CsTT12S17A 则不能。在 CsTT12 基因敲除的植株中,PA 的积累明显减少,而单体儿茶素的含量增加。此外,磷酸化修饰增强了 CsTT12 的液泡膜定位,而去磷酸化则削弱了其液泡膜定位。这项研究揭示了磷酸化在黄酮类化合物生物合成中的关键作用,并为平衡品质改善和提高抗性提供了启示。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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