Immunosensing of Cardiac Troponin I (cTnI) Using a Two-Electrode Electrochemiluminescence Platform with Near Persisting Luminescence Generated on a Ru(bpy)₃²⁺-Tripropylamine System.

IF 4.7 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-11-18 Epub Date: 2024-11-01 DOI:10.1021/acsabm.4c01247

Arathy B K Kala, Greeshma Rajeevan, Anju S Madanan, Susan Varghese, Merin K Abraham, Ali Ibrahim Shkhair, Geneva Indongo, Sony George

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引用次数: 0

Abstract

An economical, rapid, and ultrasensitive detection of biomolecules in clinical settings is very crucial, particularly for the early detection of Cardiac Troponin I (cTnI), which is the gold standard biomarker for Acute Myocardial Infarction (AMI). Electrochemiluminescence (ECL) has risen in prominence as an important technique for in vitro diagnosis and detection by virtue of its high sensitivity reaching a femtomolar level. This study introduces an economically feasible nanoplatform for ECL immunosensing, consisting of a gold nanoparticle (AuNP) with Ru(bpy)₃²⁺ and tripropylamine (TPA) system, which is a potential ECL luminophore and coreactant system. AuNPs serve the role of an ECL signal enhancer as well as the carrier of antibody, which enables the creation of a label-free immunosensor for antigen-antibody interactions. The prepared immunosensor detected cTnI with a detection limit (LOD) of 0.03 ng/mL. This potential immunosensor provides appreciable results in the detection of cTnI from spiked real serum analysis, which shows its potential application in low-resource clinical settings.

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利用在 Ru(bpy)32+-Tripropylamine 系统上产生的近持久发光的双电极电化学发光平台对心肌肌钙蛋白 I (cTnI) 进行免疫传感。

在临床环境中对生物大分子进行经济、快速和超灵敏的检测非常重要，尤其是对心肌肌钙蛋白 I（cTnI）的早期检测，它是急性心肌梗死（AMI）的金标准生物标志物。电化学发光（ECL）因其达到飞摩尔级的高灵敏度而成为体外诊断和检测的重要技术。本研究介绍了一种经济可行的 ECL 免疫传感纳米平台，它由含 Ru(bpy)32+ 的金纳米粒子（AuNP）和三丙胺（TPA）系统组成，后者是一种潜在的 ECL 发光体和核心反应物系统。AuNPs 既是 ECL 信号增强剂，又是抗体的载体，因此可以制成用于抗原-抗体相互作用的无标记免疫传感器。制备的免疫传感器可检测到 cTnI，检测限（LOD）为 0.03 ng/mL。这种潜在的免疫传感器在从加标真实血清分析中检测 cTnI 方面效果显著，显示了其在资源匮乏的临床环境中的应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.