Efficient extraction of interstitial fluid using an ultrasonic-powered replaceable hexagram-shaped hydrogel microneedle patch for monitoring of dermal pharmacokinetics and psoriatic biomarkers

IF 13.3 1区工程技术 Q1 ENGINEERING, CHEMICAL

Chemical Engineering Journal Pub Date : 2024-11-01 DOI:10.1016/j.cej.2024.157293

Shaojie Lu, Zhiming Li, Yanan Shi, Xin Wang, Hao Chang

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引用次数: 0

Abstract

Monitoring biomarkers and pharmacokinetics is crucial for the precise diagnosis and treatment of chronic skin diseases. Dermal interstitial fluid (ISF) presents an attractive alternative source of biomarkers and drugs to blood; however, a simple, efficient, and comfortable extraction method is lacking. Here, we combine high-precision 3D printing and micromolding to develop an ultrasonic-powered, replaceable hexagram-shaped hydrogel microneedle (HMN) patch. Hexagram-shaped HMNs exhibit superior mechanical strength and faster ISF extraction rates compared to cone, pyramid, and octagram shapes. The patch, attached to a holder and integrated with a commercial ultrasonic device via a customized connector, allows easy replacement after use. With ultrasound assistance, the patch extracts over 15 μL, 6 μL, and 4 μL of ISF from porcine skin ex vivo, healthy mouse skin, and psoriatic mouse skin in vivo, respectively, within 1 min. The recovered ISF, obtained via centrifugation from HMNs, is used to monitor the dermal pharmacokinetics of drugs (e.g., methotrexate) and psoriasis-related biomarkers (e.g., interleukin-17 and –22) in mouse models, showing trends comparable to those detected via skin biopsy and blood draw. This ultrasonic-powered, replaceable hexagram-shaped HMN patch holds great promise for advancing the diagnosis, treatment, and research of skin diseases.

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使用超声波驱动的可更换六角形水凝胶微针贴片高效提取组织间液，用于监测皮肤药代动力学和银屑病生物标记物

监测生物标记物和药代动力学对于精确诊断和治疗慢性皮肤病至关重要。皮肤间质液（ISF）是生物标记物和药物的一个极具吸引力的替代来源，但目前还缺乏一种简单、高效、舒适的提取方法。在这里，我们结合高精度三维打印和微成型技术，开发出一种由超声波驱动、可更换的六角形水凝胶微针（HMN）贴片。与锥形、金字塔形和八角形相比，六角形水凝胶微针具有更高的机械强度和更快的ISF提取率。这种贴片连接到支架上，并通过定制的连接器与商用超声波设备集成，使用后易于更换。在超声波辅助下，该贴片可在 1 分钟内分别从猪体外皮肤、健康小鼠皮肤和银屑病小鼠体内皮肤提取超过 15 μL、6 μL 和 4 μL 的 ISF。通过离心从 HMNs 获得的回收 ISF 可用于监测小鼠模型中药物（如甲氨蝶呤）和银屑病相关生物标记物（如白细胞介素-17 和-22）的皮肤药代动力学，其趋势与通过皮肤活检和抽血检测的趋势相当。这种由超声波驱动、可更换的六角形 HMN 贴片有望推动皮肤病的诊断、治疗和研究。

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来源期刊

Chemical Engineering Journal 工程技术-工程：化工

CiteScore

21.70

自引率

9.30%

发文量

6781

审稿时长

2.4 months

期刊介绍： The Chemical Engineering Journal is an international research journal that invites contributions of original and novel fundamental research. It aims to provide an international platform for presenting original fundamental research, interpretative reviews, and discussions on new developments in chemical engineering. The journal welcomes papers that describe novel theory and its practical application, as well as those that demonstrate the transfer of techniques from other disciplines. It also welcomes reports on carefully conducted experimental work that is soundly interpreted. The main focus of the journal is on original and rigorous research results that have broad significance. The Catalysis section within the Chemical Engineering Journal focuses specifically on Experimental and Theoretical studies in the fields of heterogeneous catalysis, molecular catalysis, and biocatalysis. These studies have industrial impact on various sectors such as chemicals, energy, materials, foods, healthcare, and environmental protection.