DNA Metabarcoding Reveals the Dietary Composition for Smooth-Coated Otter (Lutrogale perspicillata) and Asian Small-Clawed Otter (Aonyx cinereus) in Malaysia

IF 2.5 3区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES
Nursyuhada Othman, Kaviarasu Munian, Hidayah Haris, Nur Hartini Sariyati, Nurfatiha Akmal Fawwazah Abdullah-Fauzi, Mohd Lokman Ilham-Norhakim, Abd Rahman Mohd-Ridwan, Nor Rahman Aifat, Pazil Abdul-Patah, Hiroshi Sasaki, Daisuke Waku, Md Nor Shukor, Badrul Munir Md-Zain, Muhammad Abu Bakar Abdul-Latiff
{"title":"DNA Metabarcoding Reveals the Dietary Composition for Smooth-Coated Otter (Lutrogale perspicillata) and Asian Small-Clawed Otter (Aonyx cinereus) in Malaysia","authors":"Nursyuhada Othman,&nbsp;Kaviarasu Munian,&nbsp;Hidayah Haris,&nbsp;Nur Hartini Sariyati,&nbsp;Nurfatiha Akmal Fawwazah Abdullah-Fauzi,&nbsp;Mohd Lokman Ilham-Norhakim,&nbsp;Abd Rahman Mohd-Ridwan,&nbsp;Nor Rahman Aifat,&nbsp;Pazil Abdul-Patah,&nbsp;Hiroshi Sasaki,&nbsp;Daisuke Waku,&nbsp;Md Nor Shukor,&nbsp;Badrul Munir Md-Zain,&nbsp;Muhammad Abu Bakar Abdul-Latiff","doi":"10.1002/aqc.4263","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>The declining population of otter species in Malaysia, including the Asian small-clawed otter (<i>Aonyx cinereus</i>), smooth-coated otter (<i>Lutrogale perspicillata</i>) and hairy-nosed otter (<i>Lutra sumatrana</i>), is primarily attributed to habitat changes. Understanding the ecological aspects of otters, including their diets, is crucial for formulating effective conservation management strategies. To address this knowledge gap, we employed Next Generation Sequencing (NGS) as an alternative approach to enhance our understanding of the species' food chain. Specifically, this study investigated the diets of Asian small-clawed otters and smooth-coated otters by analysing an ~130 bp segment of the Cytochrome <i>b</i> (Cyt<i>b</i>) gene using Illumina Miniseq platform. The analysis successfully generated 572,311 reads, identifying four families, nine genera and 11 food species consumed by both otter species. Smooth-coated otters primarily consumed fish (96%) and frogs (4%), whereas Asian small-clawed otters exclusively fed on fish (100%), with the genus <i>Esomus</i> being the predominant fish genus found in the spraints. The diversity analysis of their diets revealed significant differences between two species (<i>p</i> &lt; 0.05). As a result, DNA metabarcoding provides a reliable method for verifying otter dietary information and can serve as a valuable tool in assessing fish diversity, thus contributing significantly to the management and conservation of otter populations, particularly in Malaysia.</p>\n </div>","PeriodicalId":55493,"journal":{"name":"Aquatic Conservation-Marine and Freshwater Ecosystems","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquatic Conservation-Marine and Freshwater Ecosystems","FirstCategoryId":"93","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/aqc.4263","RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

The declining population of otter species in Malaysia, including the Asian small-clawed otter (Aonyx cinereus), smooth-coated otter (Lutrogale perspicillata) and hairy-nosed otter (Lutra sumatrana), is primarily attributed to habitat changes. Understanding the ecological aspects of otters, including their diets, is crucial for formulating effective conservation management strategies. To address this knowledge gap, we employed Next Generation Sequencing (NGS) as an alternative approach to enhance our understanding of the species' food chain. Specifically, this study investigated the diets of Asian small-clawed otters and smooth-coated otters by analysing an ~130 bp segment of the Cytochrome b (Cytb) gene using Illumina Miniseq platform. The analysis successfully generated 572,311 reads, identifying four families, nine genera and 11 food species consumed by both otter species. Smooth-coated otters primarily consumed fish (96%) and frogs (4%), whereas Asian small-clawed otters exclusively fed on fish (100%), with the genus Esomus being the predominant fish genus found in the spraints. The diversity analysis of their diets revealed significant differences between two species (p < 0.05). As a result, DNA metabarcoding provides a reliable method for verifying otter dietary information and can serve as a valuable tool in assessing fish diversity, thus contributing significantly to the management and conservation of otter populations, particularly in Malaysia.

DNA 代谢编码揭示马来西亚平纹水獭(Lutrogale perspicillata)和亚洲小爪水獭(Aonyx cinereus)的膳食结构
马来西亚水獭物种数量的减少,包括亚洲小爪水獭(Aonyx cinereus)、平滑水獭(Lutrogale perspicillata)和毛鼻水獭(Lutra sumatrana),主要归因于栖息地的变化。了解水獭的生态方面(包括其饮食)对于制定有效的保护管理策略至关重要。为了填补这一知识空白,我们采用了下一代测序(NGS)作为一种替代方法,以加强我们对该物种食物链的了解。具体来说,本研究通过使用 Illumina Miniseq 平台分析细胞色素 b(Cytb)基因的 ~130 bp 片段,调查了亚洲小爪水獭和平滑涂层水獭的饮食。该分析成功生成了 572,311 个读数,鉴定出两种水獭食用的 4 个科、9 个属和 11 种食物。平滑纹水獭主要以鱼类(96%)和蛙类(4%)为食,而亚洲小爪水獭则完全以鱼类为食(100%),其中Esomus属是在水獭肢体中发现的主要鱼属。对其食物的多样性分析表明,两个物种之间存在显著差异(p < 0.05)。因此,DNA代谢编码提供了验证水獭饮食信息的可靠方法,可作为评估鱼类多样性的重要工具,从而为水獭种群的管理和保护做出重要贡献,尤其是在马来西亚。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Aquatic Conservation-Marine and Freshwater Ecosystems
Aquatic Conservation-Marine and Freshwater Ecosystems 环境科学-海洋与淡水生物学
CiteScore
5.50
自引率
4.20%
发文量
143
审稿时长
18-36 weeks
期刊介绍: Aquatic Conservation: Marine and Freshwater Ecosystems is an international journal dedicated to publishing original papers that relate specifically to freshwater, brackish or marine habitats and encouraging work that spans these ecosystems. This journal provides a forum in which all aspects of the conservation of aquatic biological resources can be presented and discussed, enabling greater cooperation and efficiency in solving problems in aquatic resource conservation.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信