Izargi Vega-Mas, Daniel Marino, Marlon De la Peña, Teresa Fuertes-Mendizábal, Carmen González-Murua, José María Estavillo, María Begoña González-Moro
{"title":"Enhanced photorespiratory and TCA pathways by elevated CO2 to manage ammonium nutrition in tomato leaves","authors":"Izargi Vega-Mas, Daniel Marino, Marlon De la Peña, Teresa Fuertes-Mendizábal, Carmen González-Murua, José María Estavillo, María Begoña González-Moro","doi":"10.1016/j.plaphy.2024.109216","DOIUrl":null,"url":null,"abstract":"<div><div>Plants grown under exclusive ammonium (NH<sub>4</sub><sup>+</sup>) nutrition have high carbon (C) demand to sustain proper nitrogen (N) assimilation and energy required for plant growth, generally impaired when compared to nitrate (NO<sub>3</sub><sup>−</sup>) nutrition. Thereby, the increment of the atmospheric carbon dioxide (CO<sub>2</sub>) concentration, in the context of climate change, will potentially allow plants to better face ammonium nutrition. In this work, tomato (<em>Solanum lycopersicum</em> L.) plants were grown under ammonium or nitrate nutrition in conditions of ambient (aCO<sub>2</sub>, 400 ppm) or elevated CO<sub>2</sub> (eCO<sub>2,</sub> 800 ppm) atmosphere. Elevated CO<sub>2</sub> increased photosynthesis rate and tomato shoot growth regardless of the N source. In the case of NH<sub>4</sub><sup>+</sup>-fed leaves the positive effect of elevated CO<sub>2</sub> occurred despite of the high tissue NH<sub>4</sub><sup>+</sup> accumulation. Under eCO<sub>2</sub> ammonium nutrition triggered, among others, the modulation of genes related to C provision pathways (including carbonic anhydrase and glyoxylate cycle), antioxidant response and cell membranes protection. The enhanced photosynthate production at eCO<sub>2</sub> facilitated C skeleton provision through the TCA cycle and anaplerotic pathways to promote amino acid synthesis. Moreover, photorespiratory activity was stimulated by eCO<sub>2</sub> and contributed to yield serine as additional sink for NH<sub>4</sub><sup>+</sup> excess. Overall, these changes denote a connection between the respiratory and the photorespiratory pathways linked to ammonium nutrition. This metabolic strategy may allow crops to grow efficiently using ammonium as fertilizer in a future climate change scenario, while mitigating N losses.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":null,"pages":null},"PeriodicalIF":6.1000,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology and Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0981942824008842","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Plants grown under exclusive ammonium (NH4+) nutrition have high carbon (C) demand to sustain proper nitrogen (N) assimilation and energy required for plant growth, generally impaired when compared to nitrate (NO3−) nutrition. Thereby, the increment of the atmospheric carbon dioxide (CO2) concentration, in the context of climate change, will potentially allow plants to better face ammonium nutrition. In this work, tomato (Solanum lycopersicum L.) plants were grown under ammonium or nitrate nutrition in conditions of ambient (aCO2, 400 ppm) or elevated CO2 (eCO2, 800 ppm) atmosphere. Elevated CO2 increased photosynthesis rate and tomato shoot growth regardless of the N source. In the case of NH4+-fed leaves the positive effect of elevated CO2 occurred despite of the high tissue NH4+ accumulation. Under eCO2 ammonium nutrition triggered, among others, the modulation of genes related to C provision pathways (including carbonic anhydrase and glyoxylate cycle), antioxidant response and cell membranes protection. The enhanced photosynthate production at eCO2 facilitated C skeleton provision through the TCA cycle and anaplerotic pathways to promote amino acid synthesis. Moreover, photorespiratory activity was stimulated by eCO2 and contributed to yield serine as additional sink for NH4+ excess. Overall, these changes denote a connection between the respiratory and the photorespiratory pathways linked to ammonium nutrition. This metabolic strategy may allow crops to grow efficiently using ammonium as fertilizer in a future climate change scenario, while mitigating N losses.
期刊介绍:
Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement.
Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB.
Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.