Porphyromonas gingivalis-induced autophagy exacerbates abnormal lung homeostasis: An in vivo and in vitro study

IF 2.2 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE
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引用次数: 0

Abstract

Objective

The aim of this study was to evaluate the effect of periodontal Porphyromonas gingivalis (P. gingivalis) infection on lung homeostasis and to explore the underlying mechanism.

Designs

In in vivo experiments, twelve mice were divided into two groups. The P. gingivalis infection group received P. gingivalis around the maxillary second molar, and the control group was left untreated. After 12 weeks, the histopathological changes of the lung tissue and the autophagy and apoptosis in the lung tissue cells were detected. In in vitro experiments, alveolar epithelial cell A549 was co cultured with P. gingivalis and treated with autophagy inhibitor chloroquine (CQ). Western blot was then used to detect autophagic markers LC3 and P62, and mRFP-GFP-LC3 was used to observe autophagic flux. Cell viability and apoptosis were also detected.

Results

For the in vivo experiments, pathological changes were observed in the lung tissue of the P. gingivalis infection group at 12 weeks, along with higher levels of autophagy and apoptosis in the lung tissue cells. For the in vitro experiments, infection of alveolar epithelial cells with P. gingivalis inhibited cell viability and promoted cell autophagy and apoptosis. Interestingly, we found that inhibiting P. gingivalis-activated autophagy significantly improved cell apoptosis and viability damage induced by P. gingivalis.

Conclusion

Periodontal P. gingivalis infection can cause pathological changes and abnormal homeostasis in lung tissue, and the up-regulation of autophagy induced by P. gingivalis may play a synergistic role in this process.
牙龈卟啉单胞菌诱导的自噬作用加剧了肺稳态异常:体内和体外研究
设计在活体实验中,将 12 只小鼠分为两组。牙龈弧菌感染组在上颌第二磨牙周围接受牙龈弧菌感染,对照组不做任何处理。12 周后,检测肺组织的组织病理学变化以及肺组织细胞的自噬和凋亡。在体外实验中,将肺泡上皮细胞 A549 与牙龈脓疱病菌共同培养,并用自噬抑制剂氯喹(CQ)处理。然后用 Western 印迹检测自噬标记物 LC3 和 P62,并用 mRFP-GFP-LC3 观察自噬通量。结果在体内实验中,牙龈脓疱病感染组的肺组织在 12 周后出现病理变化,肺组织细胞的自噬和凋亡水平较高。在体外实验中,牙龈弧菌感染肺泡上皮细胞会抑制细胞活力,促进细胞自噬和凋亡。有趣的是,我们发现抑制牙龈脓疱噬菌体激活的自噬作用可明显改善牙龈脓疱噬菌体诱导的细胞凋亡和活力损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Archives of oral biology
Archives of oral biology 医学-牙科与口腔外科
CiteScore
5.10
自引率
3.30%
发文量
177
审稿时长
26 days
期刊介绍: Archives of Oral Biology is an international journal which aims to publish papers of the highest scientific quality in the oral and craniofacial sciences. The journal is particularly interested in research which advances knowledge in the mechanisms of craniofacial development and disease, including: Cell and molecular biology Molecular genetics Immunology Pathogenesis Cellular microbiology Embryology Syndromology Forensic dentistry
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