Transcription factor PagERF110 inhibits leaf development by direct regulating PagHB16 in poplar

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2024-10-28 DOI:10.1016/j.plantsci.2024.112309

Zihan Cheng , Yuandong Zhu , Xinyu He , Gaofeng Fan , Jiahui Jiang , Tingbo Jiang , Xuemei Zhang

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Abstract

Ethylene-responsive factor (ERF) family genes are crucial for plant growth and development. This study analyzed the functional role of the PagERF110 gene in leaf development of Populus alba×P. glandulosa. PagERF110 contains the AP2 conserved domain and exhibits transcriptional activation activity at its C-terminus. Overexpression of PagERF110 in transgenic poplar trees resulted in reduced leaf size, leaf area, and vein xylem thickness. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) experiments confirmed that PagERF110 interacts with PagACD32.1. Transcriptome sequencing revealed that PagERF110 regulates the expression of key genes involved in leaf development. Furthermore, yeast one-hybrid (Y1H) assays, GUS staining, and ChIP experiments collectively confirmed that PagERF110 targets the expression of PagHB16. In summation, our findings demonstrate that PagERF110 functions as a negative regulator in poplar leaf development.

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转录因子 PagERF110 通过直接调控 PagHB16 抑制杨树的叶片发育

乙烯反应因子（ERF）家族基因对植物的生长和发育至关重要。本研究分析了 PagERF110 基因在白杨×腺叶植物叶片发育过程中的功能作用。PagERF110 包含 AP2 保守结构域，其 C 端具有转录激活活性。在转基因杨树中过表达 PagERF110 会导致叶片大小、叶面积和叶脉木质部厚度减小。酵母双杂交（Y2H）和双分子荧光互补（BiFC）实验证实 PagERF110 与 PagACD32.1 相互作用。转录组测序显示，PagERF110调控着参与叶片发育的关键基因的表达。此外，酵母单杂交（Y1H）实验、GUS 染色和 ChIP 实验共同证实，PagERF110 以 PagHB16 的表达为靶标。总之，我们的研究结果证明了 PagERF110 在杨树叶片发育过程中的负调控功能。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.