Screening of Diabetes-Associated Autoantigens and Serum Antibody Profiles Using a Phage Display System.

IF 2.8 Q3 MICROBIOLOGY
International Journal of Microbiology Pub Date : 2024-10-24 eCollection Date: 2024-01-01 DOI:10.1155/2024/1220644
Jun Lin, Zhenyu Wang, Hongtao Wang, Yuping Li, Yao Liu, Yige He, Qian Liu, Zichuan Chen, Yuan Ji
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引用次数: 0

Abstract

Aims/Introduction: Phage display method is a crucial tool to find novel clinically valuable diabetes-associated autoantigens and identify known autoantigen epitopes that are associated with diabetes and could provide scientific support and guidance for the artificial construction and synthesis of Type I diabetes mellitus (T1DM) novel biomarkers. Materials and Methods: The phage display system was used for the "biopanning" of T1DM serum. Following the sequencing of the phage DNAs, the homologous sequences of the above fusion heptapeptide were further investigated by BLAST to track the origin of the polypeptide sequences. The antibody spectrum revealed new T1DM-associated epitopes and antibodies. Results: A total of 1200 phage DNA were sequenced and 9 conserved polypeptide sequences were collected. It was confirmed that the zinc transporter and islet amyloid protease were among them. The conserved polypeptide sequence 8 and another three distinctive polypeptide sequences derived from Proteus were discovered. Furthermore, we expressed recombinant proteins with homologous polypeptide sequences for the human islet amyloid polypeptide (IAPP) and polypeptide precursor human zinc transporter 8 (ZNT8). Through clinical sample detection for the serum from T1DM (n = 100) and T2DM (n = 200) patients, results demonstrate the importance and relevance of these polypeptides in the recognition and classification of various forms of diabetes. Conclusion: Human pancreatic and concurrent bacterial-derived protein antigens and their epitopes were identified in this research by the phage display system, which is crucial for distinguishing different types of diabetes.

利用噬菌体展示系统筛查糖尿病相关自身抗原和血清抗体谱
目的/简介:噬菌体展示法是发现具有临床价值的新型糖尿病相关自身抗原和鉴定与糖尿病相关的已知自身抗原表位的重要工具,可为人工构建和合成 I 型糖尿病(T1DM)新型生物标志物提供科学支持和指导。材料与方法:使用噬菌体展示系统对 T1DM 血清进行 "生物筛选"。在对噬菌体 DNA 进行测序后,通过 BLAST 进一步研究了上述融合七肽的同源序列,以追踪多肽序列的来源。抗体谱显示了新的 T1DM 相关表位和抗体。研究结果共对 1200 个噬菌体 DNA 进行了测序,收集到 9 个保守的多肽序列。经证实,锌转运体和胰岛淀粉样蛋白酶也在其中。我们还发现了源自变形杆菌的保守多肽序列 8 和另外三个独特的多肽序列。此外,我们还表达了具有人类胰岛淀粉样蛋白多肽(IAPP)和人类锌转运体 8(ZNT8)多肽前体同源多肽序列的重组蛋白。通过对 T1DM(100 人)和 T2DM(200 人)患者的血清进行临床样本检测,结果表明了这些多肽在识别和分类各种糖尿病方面的重要性和相关性。结论本研究通过噬菌体展示系统鉴定了人类胰腺和并发细菌衍生蛋白抗原及其表位,这对区分不同类型的糖尿病至关重要。
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来源期刊
CiteScore
7.90
自引率
0.00%
发文量
57
审稿时长
13 weeks
期刊介绍: International Journal of Microbiology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on microorganisms and their interaction with hosts and the environment. The journal covers all microbes, including bacteria, fungi, viruses, archaea, and protozoa. Basic science will be considered, as well as medical and applied research.
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