Role of Olive Leaf Extract, Mesenchymal Stem Cells or Low Radiation Dose in Alleviating Hepatic Injury in Rats.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-09-29 eCollection Date: 2024-07-01 DOI:10.1177/15593258241289301

Ola A Gharib, Hanan A Fahmy, Fatma Y Abdou

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引用次数: 0

Abstract

Objectives: This study was conducted to determine the efficacy of mesenchymal stem cells (MSCs) or low-dose gamma radiation (LDR) on liver injury compared to the effect of olive leaf extract as a hepatoprotective agent.

Methods: Rats were allocated into six groups; group I served as the negative control. Group II received 5% dextran sodium sulfate (DSS) in its drinking water for 1 week. Group III was injected with a single dose of 1 × 10⁶ bone marrow-derived mesenchymal stem cells (BM-MSCs) intravenously. Group IV was treated as in group III after 5% DSS treatment. Group V was given 5% DSS, followed by olive leaf extract (OLE) (1000 mg/ kg, oral). Group VI: 5% DSS for 1 week, then was exposed to low-dose gamma radiation (LDR) (0.05 Gy).

Results: Rats treated with OLE, BM-MSCs, or exposed to LDR exerted significant alleviation in all hepatic biomarkers, significant enhancements in oxidative stress parameters, and improvements in inflammatory biomarkers Interleukin-1 beta (IL-1β) and Interferon gamma (INF-γ) hepatic contents compared with those of the DSS group. Histological pictures emphasized the biochemical findings.

Conclusions: BM-MSCs might be a valuable therapeutic approach to overcome hepatic injury. Exposure to LDR provided protective mechanisms that allow the body to survive better.

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橄榄叶提取物、间充质干细胞或低辐射剂量在缓解大鼠肝损伤中的作用

研究目的本研究旨在确定间充质干细胞（MSCs）或低剂量伽马射线（LDR）对肝损伤的疗效，并与作为肝保护剂的橄榄叶提取物的效果进行比较：方法：将大鼠分为六组，第一组为阴性对照组。方法：将大鼠分为 6 组，I 组为阴性对照，II 组在饮用水中加入 5% 的葡聚糖硫酸钠（DSS），为期 1 周。第 III 组大鼠静脉注射单剂量 1 × 106 骨髓间充质干细胞（BM-MSCs）。第四组在 5%DSS处理后与第三组相同。第五组：给予 5%DSS，然后给予橄榄叶提取物（OLE）（1000 毫克/千克，口服）。第六组：5%的DSS治疗1周，然后接受低剂量伽马射线（LDR）（0.05 Gy）照射：结果：与 DSS 组相比，使用 OLE、BM-间充质干细胞或暴露于 LDR 治疗的大鼠的所有肝脏生物标志物均有显著改善，氧化应激参数显著提高，炎症生物标志物白细胞介素-1β（IL-1β）和干扰素γ（INF-γ）的肝脏含量也有所改善。组织学图片证实了生化结果：结论：骨髓间充质干细胞可能是克服肝损伤的一种有价值的治疗方法。暴露于 LDR 可提供保护机制，使机体更好地存活。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464